Author:
Janetanakit Taveesak,Chaiyawong Supassama,Charoenkul Kamonpan,Tangwangvivat Ratanaporn,Chamsai Ekkapat,Udom Kitikhun,Jairak Waleemas,Amonsin Alongkorn
Abstract
Abstract
Background
Enterovirus G (EV-G) causes subclinical infections and is occasionally associated with diarrhea in pigs. In this study, we conducted a cross-sectional survey of EV-G in pigs from 73 pig farms in 20 provinces of Thailand from December 2014 to January 2018.
Results
Our results showed a high occurrence of EV-Gs which 71.6 % of fecal and intestinal samples (556/777) and 71.2 % of pig farms (52/73) were positive for EV-G by RT-PCR specific to the 5’UTR. EV-Gs could be detected in all age pig groups, and the percentage positivity was highest in the fattening group (89.7 %), followed by the nursery group (89.4 %). To characterize the viruses, 34 EV-G representatives were characterized by VP1 gene sequencing. Pairwise sequence comparison and phylogenetic analysis showed that Thai-EV-Gs belonged to the EV-G1, EV-G3, EV-G4, EV-G8, EV-G9 and EV-G10 genotypes, among which the EV-G3 was the predominant genotype in Thailand. Co-infection with different EV-G genotypes or with EV-Gs and porcine epidemic diarrhea virus (PEDV) or porcine deltacoronavirus (PDCoV) on the same pig farms was observed.
Conclusions
Our results confirmed that EV-G infection is endemic in Thailand, with a high genetic diversity of different genotypes. This study constitutes the first report of the genetic characterization of EV-GS in pigs in Thailand.
Funder
Thailand Research Fund
Chulalongkorn University
Agricultural Research Development Agency (ARDA) fund
Office of National Higher Education Science Research and Innovation Policy Council
Publisher
Springer Science and Business Media LLC
Subject
General Veterinary,General Medicine
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