Author:
Li Xiaowen,Zhang Bingzhou,Zhang Dasheng,Liu Sidang,Ren Jing
Abstract
Abstract
Background
Porcine epidemic diarrhea (PED) is a contagious intestinal disease caused by porcine epidemic diarrhea virus (PEDV) characterized by vomiting, diarrhea, anorexia, and dehydration, which have caused huge economic losses around the world. At present, vaccine immunity is still the most effective method to control the spread of PED. In this study, we have constructed a novel recombinant L. casei-OMP16-PEDVS strain expressing PEDVS protein of PEDV and OMP16 protein of Brucella abortus strain. To know the immunogenicity of the recombinant L. casei-OMP16-PEDVS candidate vaccine, it was compared with BL21-OMP16-PEDVS-F, BL21-OMP16-PEDVS, and BL21-PEDVS recombinant protein.
Results
The results showed that we could detect higher levels of IgG, neutralizing antibody, IL-4, IL-10, and INF-γ in serum and IgA in feces of L. casei-OMP16-PEDVS immunized mice, which indicated that L. casei-OMP16-PEDVS candidate vaccine could induce higher levels of humoral immunity, cellular immunity, and mucosal immunity.
Conclusion
Therefore, L. casei-OMP16-PEDVS is a promising candidate vaccine for prophylaxis of PEDV infection.
Publisher
Springer Science and Business Media LLC
Subject
General Veterinary,General Medicine
Reference34 articles.
1. Wang D, Fang L, S X. Porcine epidemic diarrhea in China. Virus Res. 2016;226:7–13.
2. Sueyoshi M, Tsuda T, Yamazaki K, Yoshida K, Nakazawa M, Sato K, et al. An immunohistochemical investigation of porcine epidemic diarrhoea. J Comp Pathol. 1995;113(1):59–67.
3. Sun RQ, Cai RJ, Chen YQ, Liang PS, Song CXJEID. Outbreak of porcine epidemic diarrhea in suckling piglets, China. Emerg Infect Dis. 2012;18(1):161–3.
4. Tong YE, Feng L, Li W. Development of bi-combined attenuated vaccine against transmissible gastroenteritis virus and porcine epidemic diarrhea virus. Chin J Prev Vet Med. 1999;21(6):35–9.
5. Pouwels PH, Leer RJ, Boersma WJ. The potential of Lactobacillus as a carrier for oral immunization: development and preliminary characterization of vector systems for targeted delivery of antigens. J Biotechnol. 1996;44(1–3):183.
Cited by
11 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献