Author:
Yan Yuzhang,Zheng Xiaohong,Wu Xueqin,Wang Ling,He Jiongjie,Hao Baocheng,Hu Tingjun,Wang Shengyi,Cui Dongan
Abstract
AbstractSalmonella infections pose a significant threat to animal and human health. Phytochemicals present a potential alternative treatment. Galla chinensis tannic acid (GCTA), a hydrolyzable polyphenolic compound, inhibits bacterial growth and demonstrates potential as an alternative or supplement to antibiotics to prevent Salmonella infections. However, little is known about the antimicrobial mechanism of GCTA against Salmonella. Here, we revealed 456 differentially expressed proteins upon GCTA treatment, impacting pathways related to DNA replication, repair, genomic stability, cell wall biogenesis, and lipid metabolism using TMT-labeled proteomic analysis. TEM analysis suggested altered bacterial morphology and structure post-treatment. A Salmonella-infected-mouse model indicated that GCTA administration improved inflammatory markers, alleviated intestinal histopathological alterations, and reduced Salmonella enterica serovar Enteritidis (S. Enteritidis) colonization in the liver and spleen of Salmonella-infected mice. The LD50 of GCTA was 4100 mg/kg with an oral single dose, vastly exceeding the therapeutic dose. Thus, GCTA exhibited antibacterial and anti-infective activity against S. Enteritidis. Our results provided insight into the molecular mechanisms of these antibacterial effects, and highlights the potential of GCTA as an alternative to antibiotics.
Funder
Innovation Program of Chinese Academy of Agricultural Sciences – Veterinary Natural Medicine and Antibiotic Replacement
National Key R&D Program of China
Basic Scientific Research Business Expenses of Central Public Welfare Scientific Research Institutes
Science and Technology Project of Lanzhou City
Technology Innovation Project of Hubei Province
Publisher
Springer Science and Business Media LLC
Cited by
1 articles.
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