Author:
Li Lin,Wang Zhenzhong,Qi Chuanxiang,Liu Shan,Gong Mingxia,Li Jinming,Wu Xiaodong,Wang Zhiliang
Abstract
Abstract
Background
Lumpy skin disease (LSD) is an acute or subacute infectious disease caused by lumpy skin disease virus (LSDV) of genus Capripoxvirus. The outbreaks of LSD were confirmed in the Yili area of the Xinjiang autonomous region in August 2019 and the Fujian province in June 2020. We detected LSDV in our daily monitoring work, then isolated, identified and sequenced the virus, and analyzed the whole genome characteristics of the isolated strain.
Results
Whole genome sequencing revealed that the strains isolated were all LSDV and were named as LSDV XJ201901 and LSDV FJ2019. The results showed that the identity based on whole genome sequences between LSDV XJ201901 and LSDV FJ2019 was 100% and the identity based on whole genome sequences between the two isolated strains and the global LSDV strains was 97.28%-99.99%, with the strain LSDV72/PrachuapKhiriKhan/Thailand/2021 (99.99%) having the highest sequence identity. Analysis of potential recombination events revealed that a total of 18 potential recombination events were identified in strains LSDV XJ201901 and LSDV FJ2019. The two strains are a recombination of Neethling vaccine LW 1959 (GeneBank: AF409138.1) with KSGP 0240 (GeneBank: KX683219.1). It was observed that Neethling vaccine LW 1959 (11/18) and KSGP 0240 (10/18) are involved in most of the potential recombination events.
Conclusions
The virus isolate in this study was LSDV and was identified as a vaccine recombinant strain. The most likely potential parent strains of the two strains in this study are Neethling vaccine LW 1959 and KSGP 0240. The strains in this study are very similar to those isolated in East and Southeast Asia since 2019.
Publisher
Springer Science and Business Media LLC
Subject
General Veterinary,General Medicine
Reference24 articles.
1. Khan YR, Ali A, Hussain K, Ijaz M, Rabbani AH, Khan RL, et al. A review: Surveillance of lumpy skin disease (LSD) a growing problem in Asia. Microb Pathog. 2021;158:105050.
2. Sprygin A, Artyuchova E, Babin Y, Prutnikov P, Kostrova E, Byadovskaya O, et al. Epidemiological characterization of lumpy skin disease outbreaks in Russia in 2016. Transbound Emerg Dis. 2018;65(6):1514–21.
3. Yanyan Z, Jingyuan Z, Jinjin Y, Jinmei Y, Xun H, Lijuan M, et al. Identification of a natural variant of African swine fever virus in China Chinese. J Vet Sci. 2021;41(02):199–207.
4. Dao TD, Tran LH, Nguyen HD, Hoang TT, Nguyen GH, Tran KVD, et al. Characterization of Lumpy skin disease virus isolated from a giraffe in Vietnam. Transbound Emerg Dis. 2022;69:e3268–72.
5. Davies FG. Observations on the epidemiology of lumpy skin disease in Kenya. J Hyg (Lond). 1982;88(1):95–102.