Detection and molecular analysis of Pseudorabies virus from free-ranging Italian wolves (Canis lupus italicus) in Italy - a case report
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Published:2024-01-03
Issue:1
Volume:20
Page:
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ISSN:1746-6148
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Container-title:BMC Veterinary Research
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language:en
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Short-container-title:BMC Vet Res
Author:
Moreno Ana,Musto Carmela,Gobbi Marco,Maioli Giulia,Menchetti Marika,Trogu Tiziana,Paniccià Marta,Lavazza Antonio,Delogu Mauro
Abstract
Abstract
Background
The only natural hosts of Pseudorabies virus (PRV) are members of the family Suidae (Sus scrofa scrofa). In mammals, the infection is usually fatal and typically causes serious neurologic disease. This study describes four Aujeszky’s disease cases in free-ranging Italian wolves (Canis lupus italicus). In Italy, the wolf is a strictly protected species and is in demographic expansion.
Case presentation
Three wolves (Wolf A, B, and C) were found in a regional park in Northern Italy, and one (Wolf D) was found in Central Italy. Wolf A and D were alive at the time of the finding and exhibited a fatal infection with epileptic seizures and dyspnoea, dying after a few hours. Wolf B presented scratching lesions under the chin and a detachment of the right earlobe, whilst Wolf C was partially eaten.
The wolves showed hepatic congestion, diffuse enteritis, moderate pericardial effusion, severe bilateral pneumonia, and diffuse hyperaemia in the brain. The diagnostic examinations included virological analyses and detection of toxic molecules able to cause serious neurological signs.
All four wolves tested positive for pseudorabies virus (PrV). The analysed sequences were placed in Italian clade 1, which is divided into two subclades, “a” and “b”. The sequences of Wolf A, B, and C were closely related to other Italian sequences in the subclade b, originally obtained from wild boars and hunting dogs. The sequence from Wolf D was located within the same clade and was closely related to the French hunting dog sequences belonging to group 4.
Conclusion
Results showed the presence of PrV strains currently circulating in wild boars and free-ranging Italian wolves. The genetic characterisation of the PrV UL44 sequences from the four wolves confirmed the close relationship with the sequences from wild boars and hunting dogs. This fact supports a possible epidemiological link with the high PrV presence in wild boars and the possibility of infection in wolves through consumption of infected wild boar carcasses or indirect transmission. To the best of our knowledge, this study is the first detection of Pseudorabies virus in free-ranging Italian wolves in northern and central Italy.
Publisher
Springer Science and Business Media LLC
Reference49 articles.
1. Steinrigl A, Revilla-Fernández S, Kolodziejek J, Wodak E, Bagó Z, Nowotny N, Schmoll F, Ko ̈ fer J. Detection and molecular characterization of Suid herpesvirus type 1 in Austrian wild boar and hunting dogs. Vet Microbiol. 2012;157(3–4):276–284. 2. Muller T, Klupp BG, Freuling C, Hoffmann B, Mojcicz M, Capua I, Palfi V, Toma B, Lutz W, Ruiz-fon F, Gortarzar C, Hlinak A, Schaarschmidt U, Zimmer K, Coraths FJ, Hahn EC, Mettenleiter TC. Characterization of pseudorabies virus of wild boar origin from Europe. Epidemiol Infect. 2010;138:1590–600. 3. Kluge JP, Beran GW, Hill HT, Platt KB. Pseudorabies (Aujeszky’s Disease). In: Leman AD, Straw BE, Mengeling WL, D’Allaire S, Taylor DJ, editors. Diseases of Swine. 7th ed. Iowa State University Press, Ames: Iowa; 1992. p. 312–23. 4. Moreno A, Chiapponi C, Sozzi E, Morelli A, Silenzi V, Gobbi M, Lavazza A, Paniccià M. Detection of a gE-deleted Pseudorabies virus strain in an Italian red fox. Vet Microbiol. 2020;244:108666. 5. Zanin E, Capua I, Casaccia C, Zuin A, Moresco A. Isolation and characterization of Aujeszky’s disease virus in captive brown bears from Italy. J Wildl Dis. 1997;33(3):632–4.
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