Bovine Digital Dermatitis: Treponema spp. on trimming equipment and chutes – effect of washing and disinfection

Author:

Ahlén Lina,Holmøy Ingrid Hunter,Sogstad Åse Margrethe,Jensen Tim Kåre,Frosth Sara,Rosander Anna,Fjeldaas Terje

Abstract

Abstract Background Digital dermatitis (DD) is a contagious bovine foot disease causing reduced animal welfare and negative economic consequences for the farmer. Treponema spp. are the most important causative agents. Studies indicate that trimming equipment can transfer DD-associated treponemes between cows. The aim of this observational study in 22 DD-positive Norwegian dairy herds was to investigate the risk of transferring Treponema spp. with trimming equipment and chutes after claw trimming, and after washing and disinfection. Swabs from the trimming equipment and chutes were collected from nine different locations, at five different time points. Bacterial DNA was extracted from 647 swabs and analysed by qPCR for Treponema spp. In addition, 172 swabs taken immediately after trimming, were analysed by a multiplex qPCR targeting T. phagedenis, T. pedis and T. medium/vincentii. Biopsy sampling from DD lesions was performed on cows in the same herds during trimming. Altogether 109 biopsies were analysed by FISH for confirmation of the DD diagnosis and identification of Treponema phylotypes (PTs). Results High numbers of Treponema spp. were detected from all nine locations on the trimming equipment and chutes immediately after trimming, and T. phagedenis was detected on two or more locations in all but two herds, 1 and 19. There was a decline in the amount of Treponema spp. after washing and disinfection. The belly belt, the cuff, and the footrest on the chute had the highest proportion of positive samples after disinfection. The belly belt had the highest copy numbers of all nine locations (median = 7.9, max = 545.1). No Treponema spp. was detected on the hoof knives after disinfection. Treponema phagedenis, T. pedis, and Treponema phylotype 3 (T. refringens) were detected by FISH analysis of the biopsies. Treponema phagedenis was detected in biopsies from all herds except 1 and 19. Conclusion This study shows that DD-associated Treponema spp. were present on the trimming equipment and chutes after trimming cows in DD-positive herds. Washing and disinfection reduced the load of Treponema spp. However, large differences in Treponema spp. between different locations were documented. High copy numbers on the grinder and the chute after disinfection, indicates that sufficient cleaning and disinfection of these locations is difficult, and that passive transfer of DD-associated treponemes (viable or not) is possible.

Funder

Animalia

Publisher

Springer Science and Business Media LLC

Reference50 articles.

1. ICAR. ICAR Claw Health Atlas International Committee for Animal Recording (ICAR). ; 2020. Available from: https://www.icar.org/ICAR_Claw_Health_Atlas.pdf.

2. Orsel K, Plummer P, Shearer J, De Buck J, Carter S, Guatteo R. Missing pieces of the puzzle to effectively control digital dermatitis. Transbound Emerg Dis. 2017;65 Suppl 1.

3. Wilson-Welder JH, Alt DP, Nally JE. Digital Dermatitis in cattle: current bacterial and immunological findings. Anim (Basel). 2015;5(4):1114–35.

4. Caddey B, Orsel K, Naushad S, Derakhshani H, De Buck J. Identification and quantification of bovine Digital Dermatitis-Associated Microbiota across Lesion stages in Feedlot Beef cattle. mSystems. 2021;6(4).

5. Rasmussen M, Capion N, Klitgaard K, Rogdo T, Fjeldaas T, Boye M, Jensen TK. Bovine digital dermatitis: possible pathogenic consortium consisting of Dichelobacter nodosus and multiple Treponema species. Vet Microbiol. 2012;160:151–61.

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3