Bioreactor level optimization of chromium(VI) reduction through Pseudomonas putida APRRJVITS11 and sustainable remediation of pathogenic DNA in water

Abstract

Abstract Background Bioremediation is one of the indispensable features of Pseudomonas putida. The use of Pseudomonas has been proved to be an effective treatment of tannery released chromium (VI). The current study is the first attempt for the optimization of chromate reduction by Pseudomonas putida strain APRRJVITS11 in an optimized bench-scale bioreactor with successful thermo-pressure elimination of the strain thereby eliminating the health risk caused by antibiotic resistant genes (ARGs). Results The growth media, modified with optimized 1.0% nitrogen, 0.5% yeast extract and 0.3% sodium, showed enhanced bacterial growth for 72 h of incubation. The optimization of aeration (1.0 vvm) and agitation (150 rpm) rates enhanced the chromate reduction by about 40% at 72 h fermentation. Thermo-pressure pathogenic DNA degradation was achieved at 90 °C and 5868 Pa for 10 min. Conclusions Successful chromium reduction and total elimination of ARGs from effluent. A two-step treatment train was proposed for chromium reduction in the environment, which should be incorporated by the existing leather industries running on conventional treatment units. Graphical Abstract