Assessment of Verbesina alternifolia and Mentha piperita oil extracts on Clinostomum phalacrocoracis metacercariae from Tilapia zillii

Abstract

Abstract Background Clinostomiasis (yellow grub disease) is a disease of freshwater fish caused by the encysted metacercariae (EMC) of Clinostomum spp. showing retarded growth, unusual host behavior, and even death in fishes. Thus, the purpose of this study was to conduct an assessment of two selected plant extract: V. alternifolia and M. piperita oil extracts on tegument surface of C. phalacrocoracis metacercariae (MC) from T.zillii as utilisation of biodegradable, eco-friendly plant extracts in environmental remediation to avoid utilization of chemotherapy to control of parasitic diseases leading to potential long-term health risks on the environment and humans. Results The results of evaluation efficacy of plant oil extracts, namely V. alternifolia and M. piperita on C. phalacrocoracis MC infecting T. zillii, were dependent on dose and exposure time. The lethal concentrations caused by V. alternifolia extract were determined LC50 at (400 ppm/24 h), and contrarily, the worms from gp2 exposed to M. piperita extract at LC50 (1000 ppm/48 h) and did not cause complete mortality among the exposed worms. Statistically, mortality of C. phalacrocoracis caused by V. alternifolia was found to be a stronger effect significantly higher than that caused by M. piperita. The fine integument structures observed suffered stronger effect that appeared as severe damage and desquamation of worm’s teguments after exposure of V. alternifolia. In contrast, the M. piperita treatment exhibited edematous, swollen teguments, and blebs. Therefore, C. phalacrocoracis was an adequate model for evaluation of in vitro anthelmintic effects, contributing to the endeavors to identify suitable plant extracts, V. alternifolia and M. piperita. Conclusions This study highlights on assessment of selected two plant extracts; V. alternifolia and M. piperita revealed a stronger effect of V. alternifolia than M. piperita on tegumental surface of C. phalacrocoracis worms and, also, recommended the successful utilization of V. alternifolia on investigated worms as anthelmintic efficacy.