Author:
Jiang Zao,Wu Wei,Qian Meng-lu
Abstract
Abstract
Background
The effect of cell injury and apoptosis induced by ultrasound with contrast agent has been verified. Contrast agent enhanced apoptosis and expression of genes that related to apoptosis and are responsive to ultrasound. This effect was associated with reactive oxygen species (ROS) production induced by the sonochemical reaction, as reported in previous studies. NF-kappa B may be one of the factors involved in oxidizing reactions or modulation during the process of ultrasound inducing apoptosis.
Results
Ultrasound irradiated gastric cancer cells (SGC7901 cell line) and hepatocellular carcinoma cells (SMMC-771 cell line) cultured in medium containing contrast agent. Significant cellular damage and apoptosis were observed in the bath cells incubated for 24 hours following 120 seconds ultrasonic irradiation. I kappa B alfa expression synchronously increased in the treatment groups of both the cell lines, and the down-regulated expression of NF-kappa B influenced its-regulated expression of genes that related to apoptosis. Production of intracellular ROS and elevation of NF-kappa B level occurred after incubation of the cells for 1 hour following ultrasonic treatment.
Conclusions
Our result suggested that contrast agent enhanced the biological effect of ultrasound. Their reaction might stimulate the transitory expression of NF-kappaB, and subsequent elevation in IκBalfa expression could lead to the apoptosis of SGC7901 cells and SMMC-771 cells.
Publisher
Springer Science and Business Media LLC
Subject
Cancer Research,Genetics,Oncology
Reference26 articles.
1. Sergeeva NS, Sviridova IK, Nikolaev AL, Ambrozevich EG, Kabisov RK, Sarantseva OS, Kurilyak OA, Al'kov SV, Sokolov VV: Effects of various modes of sanitation with low frequency ultrasound on in vitro survival of human tumor cells. Bull Exp Biol Med. 2001, 131: 279-282. 10.1023/A:1017672003969.
2. Lejbkowicz F, Salzberg S: Distinct sensitivity of normal and malignant cells to ultrasound in vitro. Environ Health Perspect. 1997, 105 (Suppl 6): 1575-1578. 10.1289/ehp.97105s61575.
3. Zhai BJ, Wu F, Shao ZY, Hu K, Zhao CL, Wang ZB: Establishment of human hepatocellular carcinoma multidrug-resistance cell line (HepG2/Adm) and study apoptosis induced by low-frequency pulse ultrasound exposure. Zhonghua Gan Zang Bing Za Zhi. 2004, 12: 95-98.
4. Fuciarelli AF, Sisk EC, Thomas RM, Miller DL: Induction of base damage in DNA solutions by ultrasonic cavitation. Free Radic Biol Med. 1995, 18: 231-238. 10.1016/0891-5849(94)00119-5.
5. Cheng Q, Qian ML: Low frequency dynamic behavior of ultrasound microbubble contrast agent. Tech Acoust. 2006, 25: 292-298.
Cited by
11 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献