Author:
Azike Chike Godwin,Charpentier Paul Abrahams,Hou Jirui,Pei Hua,King Lui Edmund Man
Abstract
Abstract
Background
Immuno-modulatory effects of ginseng, including both immuno-stimulatory and immuno-suppressive effects, have been widely reported. This study aims to determine whether the paradoxical immuno-modulatory effect is related to unique phytochemical profiles of different North American (NA) ginseng, namely aqueous (AQ) and alcoholic (ALC) extracts.
Methods
AQ and ALC extracts were prepared and their immuno-bioactivity were studied in vitro in murine macrophages (Raw 264.7) through measuring the direct stimulatory production of pro-inflammatory mediator and cytokines as well as the suppression of lipopolysaccharide (LPS)-stimulatory response by the two extracts. Gel permeation chromatography was used to fractionate and isolate phytochemicals for characterization of ginseng extracts.
Results
AQ extract up-regulated the production of nitric oxide (NO), tumour necrosis factor-α (TNF-α) and interleukin-6 (IL-6) while ALC extract did not. ALC extract but not AQ extract suppressed LPS-induced macrophage NO and TNF-α production. These immuno-stimulatory and suppressive effects were exhibited at similar extract concentrations. Moreover, the macrophage-stimulating activity of the AQ extract was inhibited in the presence of ALC extract. Fractionation of AQ extract revealed the presence of two major peaks at 230 nm with average molecular weights of 73,000 and 37,000 Da. The first fraction had similar elution volume as the crude polysaccharide (PS) fraction isolated from the AQ extract, and it was the only bioactive species. Parallel fractionation study of ALC extract yielded similar elution profiles; however, both sub-fractions were devoid of PS. Fraction I of the ALC extract suppressed LPS-induced NO production dose-dependently.
Conclusion
ALC extract of NA ginseng, which was devoid of PS, was immuno-inhibitory whereas the AQ extract, which contained PS, was immuno-stimulatory. These extract-related anti-inflammatory and pro-inflammatory effects may be considered as the Yin and Yang actions of ginseng.
Publisher
Springer Science and Business Media LLC
Subject
Complementary and alternative medicine,Pharmacology
Reference28 articles.
1. Angelova N, Kong HW, van der Heijden R, Yang SY, Choi YH, Kim HK, Wang M, Hankemeier T, van der Greef J, Xu G, Verpoorte R: Recent methodology in phytochemical analysis of ginseng. Phytochem Anal. 2008, 19: 2-16. 10.1002/pca.1049.
2. Borchers AT, Keen CL, Stern JS, Gershwin ME: Inflammation and native American medicine: the role of botanicals. Am J Clin Nutr. 2000, 72: 339-47.
3. Agriculture and Agri-Food Canada, Overview of the Canadian Special Crops Industry - Ginseng. [http://www.ats-sea.agr.gc.ca/can/4752-eng.htm#j]
4. Health Canada Drugs and Health Products, Natural Health Product Monograph Panax Ginseng. [http://www.hc-sc.gc.ca/dhp-mps/alt_formats/pacrb-dgapcr/pdf/prodnatur/applications/licen-prod/monograph/mono_panax_ginseng-eng.pdf]
5. Attele AS, Wu JA, Yuan CS: Ginseng pharmacology; multiple constituents and multiple actions. Biochem Pharmacol. 1999, 58: 1685-1693. 10.1016/S0006-2952(99)00212-9.
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