Author:
Liu Wen-Bei,Wang He-Li,Chen Lei,Tang Biao,Ke Guolin,Wang Shuai,Sun Yin-Qiao,Ma Junting,Lyu Da-Lun
Abstract
Abstract
Background
Melanoma is among the most aggressive types of skin malignancy and can have an unpredictable clinical course. Exploration of novel therapeutic targets and their regulators remains essential for the prevention and treatment of melanoma.
Methods
HSDL2 protein levels were examined by immunohistochemistry. The roles of HSDL2 in cell proliferation and apoptosis were identified by CCK-8 and colony formation assays. The function of HSDL2 in cell apoptosis was analysed by flow cytometry. Western blotting, cell proliferation and apoptosis and a xenograft tumour model were utilized to explore the inhibitory functions and mechanisms of CuE in melanoma.
Results
HSDL2 is overexpressed in melanoma and promotes melanoma progression by activating the ERK and AKT pathways. CuE could inhibit the ERK and AKT pathways by decreasing HSDL2 expression; therefore, CuE could inhibit melanoma growth in vitro and in vivo.
Conclusion
HSDL2 may be a promising therapeutic target against melanoma, and CuE can inhibit melanoma by downregulating HSDL2 expression.
Funder
Innovative Research Group Project of the National Natural Science Foundation of China
the Colleges and Universities in Anhui Province Natural Science Research Projects
Grants for Scientific Research of BSKY from Anhui Medical University
the Foundation of the Key Laboratory of Colleges and Universities in Anhui Province
the Research Fund of Anhui Medical University
Publisher
Springer Science and Business Media LLC
Subject
Complementary and alternative medicine,Pharmacology
Reference36 articles.
1. Sun FD, Wang PC, Shang J, Zou SH, Du X. Ibrutinib presents antitumor activity in skin cancer and induces autophagy. Eur Rev Med Pharmacol Sci. 2018;22(2):561–6.
2. Carr S, Smith C, Wernberg J. Epidemiology and risk factors of melanoma. Surg Clin N Am. 2020;100(1):1–12.
3. Li C, Zhang Q, Li Z, Feng S, Luo H, Liu R, Wang L, Geng Y, Zhao X, Yang Z, et al. Efficacy and safety of carbon-ion radiotherapy for the malignant melanoma: a systematic review. Cancer Med. 2020;9(15):5293–305.
4. Zhou H, Wu J, Leng S, Hou C, Mo L, Xie X, Wang L, Xu Y. Knockdown of circular RNA VANGL1 inhibits TGF-beta-induced epithelial-mesenchymal transition in melanoma cells by sponging miR-150–5p. J Cell Mol Med. 2021;25:10827.
5. Miller AJ, Mihm MC Jr. Melanoma. N Engl J Med. 2006;355(1):51–65.
Cited by
15 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献