Abstract
Abstract
Background
Ganoderma sp., such as Ganoderma tsugae (GT), play an important role in traditional Chinese medicine. Ganoderma sp. contains several constituents, including Sacacchin, which has recently drawn attention because it can not only enhance the repair of muscle damage but also strengthen the muscle enforcement. Although Ganoderma sp. have a therapeutic effect for neuromuscular disorders, the underlying mechanism remains unclear. This study investigated the effect and underlying molecular mechanism of micronized sacchachitin (mSC) on satellite cells (SCs), which are known as the muscle stem cells.
Methods
The myogenic cells, included SCs (Pax7+) were isolated from tibialis anterior muscles of a healthy rat and were cultured in growth media with different mSC concentrations. For the evaluation of SC proliferation, these cultivated cells were immunostained with Pax7 and bromodeoxyuridine assessed simultaneously. The molecular signal pathway was further investigated by using Western blotting and signal pathway inhibitors.
Results
Our data revealed that 200 µg/mL mSC had an optimal capability to significantly enhance the SC proliferation. Furthermore, this enhancement of SC proliferation was verified to be involved with activation of TAK1-JNK-AP-1 signaling pathway through TLR2, whose expression on SC surface was confirmed for the first time here.
Conclusion
Micronized sacchachitin extracted from GT was capable of promoting the proliferation of SC under a correct concentration.
Funder
Taipei Medical University Hospital
Publisher
Springer Science and Business Media LLC
Subject
Complementary and alternative medicine,Pharmacology
Reference44 articles.
1. Pang X, Chen Z, Gao X, Liu W, Slavin M, Yao W, Yu LL. Potential of a novel polysaccharide preparation (GLPP) from Anhui-grown Ganoderma lucidum in tumor treatment and immunostimulation. J Food Sci. 2007;72(6):S435–42.
2. Su CH, Sun CS, Juan SW, Hu CH, Ke WT, Sheu MT. Fungal mycelia as the source of chitin and polysaccharides and their applications as skin substitutes. Biomaterials. 1997;18(17):1169–74.
3. Chien RC, Yen MT, Tseng YH, Mau JL. Chemical characteristics and anti-proliferation activities of Ganoderma tsugae polysaccharides. Carbohydr Polym. 2015;128:90–8.
4. Chen ML, Hsieh CC, Chiang BL, Lin BF. Triterpenoids and polysaccharide fractions of Ganoderma tsugae exert different effects on antiallergic activities. Evid Based Complement Alternat Med. 2015;2015:754836.
5. Zjawiony JK. Biologically active compounds from Aphyllophorales (polypore) fungi. J Nat Prod. 2004;67(2):300–10.
Cited by
5 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献