Resistant starch (RS), a novel endogenous inert marker for detecting glucose absorption of small intestine with sweeteners administration in mice

Author:

Wu Yaran,Cai Lei,Xie Xingzi,Yang Shuying,Shi Qing,Jia Hongzhe,Gu Xuqiang,Deng Jingmin,Shi Mingzhao,Chen Qiuping,Cao Shaoqian,Cai Shuangfeng

Abstract

AbstractResistant starch could be degraded by the fermentation of colonic microorganisms in the large intestine of mammals, but not in the small intestine. In this study, we established a novel strategy by using resistant starch as an endogenous marker to determine the glucose absorption of the small intestine of laboratory animals. By optimization of the classical enzymatic method of starch measurement, the demand for the sample weight was reduced by 90%. Moreover, the amount of resistant starch in normal feed was detectable without any extra addition. The value of small intestine glucose absorption of mice was similar when using resistant starch and titanium dioxide as inert markers. The fermentation of resistant starch by intestinal microorganisms in the small intestine was demonstrated not disturbing the detection of glucose absorption significantly. Artificial sweeteners exposed ICR mice showed different glucose absorption which indicated, first, resistant starch can be used as a novel endogenous marker in the small intestine of small animals; second, although glucose tolerance did not change in mice after short-term exposure to artificial sweeteners, there were significant changes in glucose absorption associated with it; third, the short-term exposure resulted in no significant change in glucose tolerance.

Funder

General projects of Department of Education of Zhejiang Province

National Natural Science Foundation of China

Quanzhou City Science & Technology Program of China

Fundamental Special Funds for the Provincial Universities of Zhejiang

Zhejiang Wanli University Research & Development Funding

Publisher

Springer Science and Business Media LLC

Subject

Organic Chemistry,General Biochemistry, Genetics and Molecular Biology

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