Fluorescence strip sensor for sensitive determination of prometryn in foods

Abstract

AbstractWe report the development of a sensitive, fast, and simple method for the detection of prometryn (PRO) in potato and celery using a lateral flow immunochromatographic assay (LFIA). A monoclonal antibody (mAb) against PRO was prepared under optimized conditions, and the 50% inhibition concentration (IC50) for use in ELISA was determined to be 0.6 ng mL−1. Moreover, the cut-off value for use in an LFIA was 50 ng mL−1. To validate the reliability of the developed LFIA strip, real samples were used, and the recovery rate was found to be 99.3–104.3%. The assays were completed within 8 min, demonstrating the suitability of our developed methods for the detection of PRO in potato and celery.