Generation of GHR-modified pigs as Laron syndrome models via a dual-sgRNAs/Cas9 system and somatic cell nuclear transfer

Author:

Yu Honghao,Long Weihu,Zhang Xuezeng,Xu Kaixiang,Guo Jianxiong,Zhao Heng,Li Honghui,Qing Yubo,Pan Weirong,Jia Baoyu,Zhao Hong-Ye,Huang Xingxu,Wei Hong-Jiang

Funder

the National Genetically Modified Organisms Breeding Major Projects

the National Natural Science Foundation of China

Innovative Research Team of Science and Technology in Yunnan Province

Innovative Research Team (in Science and Technology) in University of Yunnan Province

Major Program on Basic Research Projects of Yunnan Province

Publisher

Springer Science and Business Media LLC

Subject

General Biochemistry, Genetics and Molecular Biology,General Medicine

Reference39 articles.

1. Laron Z, Pertzelan A, Mannheimer S. Genetic pituitary dwarfism with high serum concentation of growth hormone–a new inborn error of metabolism? Isr J Med Sci. 1966;2:152–5.

2. Ghizzoni L, Duquesnoy P, Torresani T, Vottero A, Goossens M, Bernasconi S. Isolated growth hormone deficiency type IA associated with a 45-kilobase gene deletion within the human growth hormone gene cluster in an Italian family. Pediatr Res. 1994;36:654–9.

3. Woods KA, Fraser NC, Postel-Vinay MC, Savage MO, Clark AJ. A homozygous splice site mutation affecting the intracellular domain of the growth hormone (GH) receptor resulting in Laron syndrome with elevated GH-binding protein. J Clin Endocrinol Metab. 1996;81:1686–90.

4. Li F, Li Y, Liu H, Zhang X, Liu C, Tian K, Bolund L, Dou H, Yang W, Yang H, et al. Transgenic Wuzhishan minipigs designed to express a dominant-negative porcine growth hormone receptor display small stature and a perturbed insulin/IGF-1 pathway. Transgenic Res. 2015;24:1029–42.

5. Fintini D, Brufani C, Cappa M. Profile of mecasermin for the long-term treatment of growth failure in children and adolescents with severe primary IGF-1 deficiency. Ther Clin Risk Manag. 2009;5:553–9.

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