Tyrosine phosphorylation of band 3 impairs the storage quality of suspended red blood cells in the Tibetan high-altitude polycythemia population

Author:

Wu Xiaodong,Liu Zhijuan,Hao Doudou,Zhao Qin,Li Wanjing,Xie Maodi,Feng Xia,Liao Xia,Chen Siyuan,Wang Siyu,Zhou Chaohua,Long Wenchun,Zhong Yajun,Li Shen,Cao Ye,Wang Hong,Wang Aiping,Xu Yuehong,Huang Min,Liu Jiaxin,Zhong Rui,Wu Yunhong,He ZengORCID

Abstract

AbstractDue to environmental hypoxia on the Tibetan Plateau, local residents often exhibit a compensative increase in hemoglobin concentration to maintain the body’s oxygen supply. However, increases in hemoglobin and hematocrit (Hct) pose a serious challenge to the quality of stored suspended red blood cells (SRBCs) prepared from the blood of high-hemoglobin populations, especially populations at high altitude with polycythemia in Tibet. To explore the difference in storage quality of SRBCs prepared from plateau residents with a high hemoglobin concentration, blood donors were recruited from Tibet (> 3600 m) and Chengdu (≈ 500 m) and divided into a high-altitude control (HAC) group, high-altitude polycythemia (HAPC) group and lowland control (LLC) group according to their hemoglobin concentration and altitude of residence. The extracellular acidification rate (ECAR), pyruvate kinase (PK) activity and band 3 tyrosine phosphorylation were analyzed on the day of blood collection. Then, whole-blood samples were processed into SRBCs, and storage quality parameters were analyzed aseptically on days 1, 14, 21 and 35 of storage. Overall, we found that tyrosine 21 phosphorylation activated glycolysis by releasing glycolytic enzymes from the cytosolic domain of band 3, thus increasing glucose consumption and lactate accumulation during storage, in the HAPC group. In addition, band 3 tyrosine phosphorylation impaired erythrocyte deformability, accompanied by the highest hemolysis rate in the HAPC group, during storage. We believe that these results will stimulate new ideas to further optimize current additive solutions for the high-hemoglobin population in Tibet and reveal new therapeutic targets for the treatment of HAPC populations.

Funder

the science and technology department of Tibet, the central government guides the local science and technology development fund project

Sichuan Province Science and Technology Support Program

Science and Technology Plan Projects of Tibet Autonomous Region

Natural Science Foundation of Tibet Autonomous Region

Young Scientists Fund

Medical and Health Science and Technology Innovation Project of Chinese Academy of Medical Sciences

Projects of Hospital of Chengdu Office of People’s Government of Tibetan Autonomous Region

Publisher

Springer Science and Business Media LLC

Subject

General Biochemistry, Genetics and Molecular Biology,General Medicine

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