Author:
Gou Xin,Wu Jing,Huang Mingqing,Weng Yuting,Yang Tongxin,Chen Tao,Li Guiqing,Fang Kewei
Abstract
Abstract
Background
Diabetic bladder disease is common complications of diabetes, its
symptoms are diverse, can be due to different stages. In this study we investigate
the mechanism of miR-128 targeting CB1 expression to mediate the occurrence of
diabetic bladder disease.
Methods
Bioinformatics analysis predicts related regulatory factors of miR-128
in diabetic bladder disease. Models of diabetic bladder lesions were constructed in
male SD rats by intraperitoneal injection of streptozotocin at 65 mg/kg body weight.
The expression of miR-128 and CB1 mRNA in bladder tissues of each group was detected
by RT-qPCR, and CB1, NF-KB, p-JNK and Bcl2 protein expression was detected by Western
Blotting. We tested the function of the bladder by urodynamics, detected the
pathological characteristics of the bladder tissue by HE staining, and verified the
targeting relationship between miR-128 and CB1 through the prediction of the
biological website, dual luciferase reporter gene assay and RIP.
Results
miR-128 was highly expressed in the bladder tissue of diabetic rats.
Inhibition of miR-128 could improve the occurrence of diabetic bladder lesions in
rats. miR-128 could target the inhibition of CB1 expression, and high expression of
CB1 could antagonize miR-128 against diabetic bladder. In the diabetic bladder,
miR-128 can regulate the expression of NF-KB and p-JNK through CB1 and affect the
level of apoptosis. miR-128 regulates NF-KB/p-JNK through CB1, thus affecting the
occurrence of diabetic bladder disease.
Conclusion
The high expression of miR-128 can down-regulate the expression of CB1,
promote the activation of NF-KB and p-JNK, increase the level of apoptosis and
promote the occurrence of diabetic bladder disease.
Funder
the Yunnan Provincial Science and Technology Department
National Natural Science
Foundation of China
Medical Discipline Leaders of Health and Family Planning Commission of
Yunnan Province
Young and Middle-Aged Academic and Technical Leaders Reserved Scholar of
Yunnan Province
the Engineering and Research Center of Yunnan College and University for
Female Pelvic Floor Disease Diagnosis and Treatment
Publisher
Springer Science and Business Media LLC
Subject
General Biochemistry, Genetics and Molecular Biology,General Medicine
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