Increased Leishmania infantum resistance to miltefosine and amphotericin B after treatment of a dog with miltefosine and allopurinol

Author:

Gonçalves GustavoORCID,Campos Monique Paiva,Gonçalves Alessandra Silva,Medeiros Lia Carolina Soares,Figueiredo Fabiano Borges

Abstract

Abstract Background Leishmania infantum is the most important etiological agent of visceral leishmaniasis in the Americas and Mediterranean region, and the dog is the main host. Miltefosine was authorized to treat canine leishmaniasis (CanL) in Brazil in 2017, but there is a persistent fear of the emergence of parasites resistant not only to this drug but, through cross-resistance mechanisms, also to meglumine antimoniate and amphotericin B. Additionally, the literature shows that acquisition of resistance is followed by increased parasite fitness, with higher rates of proliferation, infectivity and metacyclogenesis, which are drivers of parasite virulence. In this context, the aim of this study was to analyze the impact of treating a dog with miltefosine and allopurinol on the generation of parasites resistant to miltefosine, amphotericin B and meglumine antimoniate. Methods In vitro susceptibility tests were conducted against miltefosine, amphotericin B and meglumine antimoniate with T0 (parasites isolated from a dog before treatment with miltefosine plus allopurinol), T1 (after 1 course of treatment) and T2 (after 2 courses of treatment) isolates. The rates of cell proliferation, infectivity and metacyclogenesis of the isolates were also evaluated. Results The results indicate a gradual increase in parasite resistance to miltefosine and amphotericin B with increasing the number of treatment courses. An increasing trend in the metacyclogenesis rate of the parasites was also observed as drug resistance increased. Conclusion The data indicates an increased L. infantum resistance to miltefosine and amphotericin B after the treatment of a dog with miltefosine plus allopurinol. Further studies with a larger number of L. infantum strains isolated from dogs with varied immune response profiles and undergoing different treatment regimes, are advocated. Graphical Abstract

Funder

conselho nacional de desenvolvimento científico e tecnológico

fundação oswaldo cruz

Publisher

Springer Science and Business Media LLC

Subject

Infectious Diseases,Parasitology

Reference33 articles.

1. Dantas-Torres F. Canine leishmaniosis in South America. Parasit Vectors. 2009;2(Suppl 1):S1.

2. Departamento de vigilância epidemiológica, Secretaria de Vigilância em Saúde, Ministério da Saúde (BR). Guia de Vigilância em Saúde. 3rd ed. Brasilia: Ministério da Saúde (BR). 2019.

3. Werneck GL. Visceral leishmaniasis in Brazil: rationale and concerns related to reservoir control. Rev Saude Publica. 2014;48:851–5.

4. Ministério da Agricultura, Pecuária e Abastecimento e Ministério da Saúde. Nota técnica conjunta no. 001/2016. https://www.sbmt.org.br/portal/wp-content/uploads/2016/09/nota-tecnica.pdf. Accessed 18 Sep 2021.

5. dos Santos NF, Avino VC, Galvis-Ovallos F, Pereira-Chioccola VL, Moreira MAB, Romariz APPL, et al. Use of miltefosine to treat canine visceral leishmaniasis caused by Leishmania infantum in Brazil. Parasit Vectors. 2019;12:79.

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