Author:
Debalke Serkadis,Habtewold Tibebu,Christophides George K.,Duchateau Luc
Abstract
Abstract
Background
Malaria vector mosquitoes acquire midgut microbiota primarily from their habitat. The homeostasis of these microbial communities plays an essential role in the mosquito longevity, the most essential factor in the mosquito vectorial capacity. Our recent study revealed that silencing genes involved in regulation of the midgut homeostasis including FN3D1, FN3D3 and GPRGr9 reduced the survival of female adult Anopheles arabiensis mosquitoes. In the present study, we investigate the stability of the gene silencing efficiency of mosquitoes reared in three different breeding conditions representing distinct larval habitat types: town brick pits in Jimma, flood pools in the rural land of Asendabo and roadside pools in Wolkite.
Methods
First-instar larvae of An. arabiensis mosquitoes were reared separately using water collected from the three breeding sites. The resulting adult females were micro-injected with dsRNA targeting the FN3D1 gene (AARA003032) and their survival was monitored. Control mosquitoes were injected with dsRNA Lacz. In addition, the load of midgut microbiota of these mosquitoes was determined using flow cytometry.
Results
Survival of naïve adult female mosquitoes differed between the three sites. Mosquitoes reared using water collected from brick pits and flood pools survived longer than mosquitoes reared using water collected from roadside. However, the FN3D1 gene silencing effect on survival did not differ between the three sites.
Conclusions
The present study revealed that the efficacy of FN3D1 gene silencing is not affected by variation in the larval habitat. Thus, silencing this gene has potential for application throughout sub-Saharan Africa.
Funder
Institutional University Cooperation IUC-JU
Publisher
Springer Science and Business Media LLC
Subject
Infectious Diseases,Parasitology
Reference41 articles.
1. WHO. World malaria report. 2018. Accessed June 2019.
2. Gillies MT, Wilkes TJ. A study of the age-composition of populations of Anopheles gambiae giles and Anopheles funestus Giles in north-eastern Tanzania. Bull Entomol Res. 1965;56:237–62.
3. Service MW, Towson H. The Anopheles vector. In: Warrell DA, Gilles HM, editors. Essential malariology. London: Arnold; 2002. p. 59–84.
4. Smith DL, McKenzie EF. Statics and dynamics of malaria infection in Anopheles mosquitoes. Malar J. 2004;3:13.
5. Oyewole IO, Momoh OO, Anyasor GN, Ogunnowo AA, Ibidapo CA, Oduola OA, et al. Physico-chemical characteristics of Anopheles breeding sites: impact on fecundity and progeny development. Afr J Environ Sci Technol. 2009;3:447–52.
Cited by
1 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献