Author:
Haut Maja,Król Nina,Obiegala Anna,Seeger Johannes,Pfeffer Martin
Abstract
Abstract
Background
Ixodes spp. are vectors of zoonotic pathogens. All three active life stages (larvae, nymphs, adults) need to feed on a host in order to develop. Usually ticks parasitize attached to the external surface of their hosts’ skin. Interestingly, in some cases ticks can also be found in the subcutaneous tissue in a variety of hosts, such as red foxes (Vulpes vulpes), raccoon dogs (Nyctereutes procyonoides) and dogs.
Methods
The visceral side of 126 red fox-furs from Germany was examined visually searching for ticks. The localization of ticks was recorded and assigned to ten specific body parts. Morphological identification of ticks was performed according to standardized taxonomic protocols. Ticks which could not be further identified were examined genetically via conventional PCR targeting the 16S rRNA and cox1 gene. Hematoxylin and eosin (H&E) staining was used for histopathological examination.
Results
In 111 out of 126 (88.1%) examined coats, at least one tick was found in the subcutaneous tissue. A total of 1203 ticks were removed from the subcutaneous tissue. Well-preserved ticks could be identified based on morphological criteria, but most ticks were in a progressed state of decomposition. Here, morphological species identification was not successful. Also, PCR methods did not lead to a successful species identification. The following species and development stages were found by morphological identification: Ixodes ricinus (female, n = 289; male, n = 8; nymph, n = 1), I. hexagonus (female, n = 2), I. canisuga (female, n = 1). Male I. ricinus were found individually or copulating in pairs with females. Subcutaneous ticks were localized at three predominant affected body parts: ears, axillar and inguinal region. Histological examination of subcutaneous ticks revealed a granulomatous panniculitis.
Conclusions
To the authors’ knowledge, this is the first finding of highly prevalent subcutaneous ticks in red foxes from Germany. Subcutaneous location of ticks seems to be very common in red foxes and the rule rather than the exception. Deep embedment of longirostra and long feeding times of females seem to put the subcutaneous location in favor. Most foxes were infested in the inguinal area, where the skin is thin and less hairy.
Publisher
Springer Science and Business Media LLC
Subject
Infectious Diseases,Parasitology
Reference37 articles.
1. Oliver JH. Biology and systematics of ticks (Acari:Ixodida). Annu Rev Ecol Syst. 1989;20:397–430.
2. Stanek G. Büchse der Pandora: Krankheitserreger in Ixodes ricinus-Zecken in Mitteleuropa. Wien Klin Wochenschr. 2009;121:673–83.
3. Cornely M, Schultz U. Zur Zeckenfauna Ostdeutschlands. Angew Parasitol. 1992;33:173–83.
4. Heidrich J. Prevalence of Borrelia burgdorferi sensu lato in red foxes (Vulpes vulpes) in eastern Brandenburg. Berlin: PhD Thesis, Freie Universität Berlin; 2000.
5. Liebisch A, Walter G. Ticks of domestic and wild animals in Germany: on the occurrence and biology of the hedgehog tick (Ixodes hexagonus) and the fox tick (Ixodes canisuga). Deutsche Tierärztliche Wochenschrift. 1986;93:447–50.
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