Characterization of a d-lyxose isomerase from Bacillus velezensis and its application for the production of d-mannose and l-ribose

Abstract

Abstractd-Mannose andl-ribose are two important monosaccharides, which have attracted public attention recently because of their great application potentials in food, cosmetic and pharmaceutical industries. Sugar isomerases catalyze the sugar isomerization and therefore can be used as the biocatalysts for production of the high-value sugars from inexpensive sugars.l-arabinose isomerase catalyzes the conversion ofl-arabinose tol-ribulose, whiled-lyxose isomerase catalyzesl-ribulose andd-fructose tol-ribose andd-mannose, respectively. In this paper, a putatived-LI fromBacillus velezensis(BvLI) was identified, characterized and used to produced-mannose andl-ribose fromd-fructose andl-arabinose, respectively. The recombinant BvLI exhibited a maximum activity at 55 °C and pH 6.5, in the presence of 0.1 mM Co2+. Approximately 110.75 g/Ld-mannose was obtained from 500 g/Ld-fructose in 6 h by the recombinant BvLI, and approximately 105 g/Ll-ribose was obtained from 500 g/Ll-arabinose in 8 h by the successive biocatalysis ofl-arabinose isomerase fromBacillus licheniformis(BlAI) and BvLI.