Author:
Tao Yongfu,Jiang Lu,Liu Qingqing,Zhang Yan,Zhang Rui,Ingvardsen Christina Roenn,Frei Ursula Karoline,Wang Baobao,Lai Jinsheng,Lübberstedt Thomas,Xu Mingliang
Abstract
Abstract
Background
Sugarcane mosaic virus (SCMV) disease causes substantial losses of grain yield and forage biomass in susceptible maize cultivars. Maize resistance to SCMV is associated with two dominant genes, Scmv1 and Scmv2, which are located on the short arm of chromosome 6 and near the centromere region of chromosome 3, respectively. We combined both linkage and association mapping to identify positional candidate genes for Scmv1.
Results
Scmv1 was fine-mapped in a segregating population derived from near-isogenic lines and further validated and fine-mapped using two recombinant inbred line populations. The combined results assigned the Scmv1 locus to a 59.21-kb interval, and candidate genes within this region were predicted based on the publicly available B73 sequence. None of three predicted genes that are possibly involved in the disease resistance response are similar to receptor-like resistance genes. Candidate gene–based association mapping was conducted using a panel of 94 inbred lines with variable resistance to SCMV. A presence/absence variation (PAV) in the Scmv1 region and two polymorphic sites around the Zmtrx-h gene were significantly associated with SCMV resistance.
Conclusion
Combined linkage and association mapping pinpoints Zmtrx-h as the most likely positional candidate gene for Scmv1. These results pave the way towards cloning of Scmv1 and facilitate marker-assisted selection for potyvirus resistance in maize.
Publisher
Springer Science and Business Media LLC
Reference53 articles.
1. Fuchs E, Gruntzig M: Influence of sugarcane mosaic-virus(SCMV) and maize-dwarf mosaic-virus(MDMV) on the growth and yield of 2 maize varieties. Zeitschrift Fur Pflanzenkrankheiten Und Pflanzenschutz-Journal of Plant Diseases and Protection. 1995, 102 (1): 44-50.
2. Wang ZH, Li XH, Yuan LX, Han XQ, Zhang SH: Genetic diversity of elite maize germplasm for resistantance to SCMV. Acta Agron Sin. 2003, 29 (3): 391-396.
3. Trzmiel K: First report of sugarcane mosaic virus infecting maize in Poland. Plant Dis. 2009, 93 (10): 1078-1078.
4. Marie-Jeanne V, Hariri D, Doucet R, Signoret PA: First report of sugarcane mosaic virus on maize in the centre region of France. Plant Dis. 2011, 95 (1): 70-70.
5. Shukla DD, Ward CW: Identification and classification of potyviruses on the basis of coat protein-sequence data and serology. Arch Virol. 1989, 106 (3–4): 171-200.
Cited by
65 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献