Author:
Derbyshire Paul,McCann Maureen C,Roberts Keith
Abstract
Abstract
Background
Cell elongation is mainly limited by the extensibility of the cell wall. Dicotyledonous primary (growing) cell walls contain cellulose, xyloglucan, pectin and proteins, but little is known about how each polymer class contributes to the cell wall mechanical properties that control extensibility.
Results
We present evidence that the degree of pectin methyl-esterification (DE%) limits cell growth, and that a minimum level of about 60% DE is required for normal cell elongation in Arabidopsis hypocotyls. When the average DE% falls below this level, as in two gibberellic acid (GA) mutants ga1-3 and gai, and plants expressing pectin methyl-esterase (PME1) from Aspergillus aculeatus, then hypocotyl elongation is reduced.
Conclusion
Low average levels of pectin DE% are associated with reduced cell elongation, implicating PMEs, the enzymes that regulate DE%, in the cell elongation process and in responses to GA. At high average DE% other components of the cell wall limit GA-induced growth.
Publisher
Springer Science and Business Media LLC
Reference77 articles.
1. Carpita NC, Gibeaut DM: Structural models of primary cell walls in flowering plants - consistency of molecular structure with the physical properties of the walls during growth. Plant J. 1993, 3 (1): 1-30. 10.1046/j.1365-313X.1993.00999.x.
2. McCann MC, Roberts K: Architecture of the primary cell wall. The Cytoskeletal Basis of Plant Growth and Form. Edited by: Lloyd C. London , Academic Press, 1991:109-129.
3. Green PB: Organogenesis - a biophysical view. Annu Rev Plant Physiol Plant Molec Biol. 1980, 31: 51-82. 10.1146/annurev.pp.31.060180.000411.
4. Brummell DA, Lashbrook CC, Bennett AB: Enzymatic Conversion of Biomass for Fuels Production. 1994, 566: 100-129. Plant endo-1,4-beta-D-glucanases - structure, properties, and physiological function Acs Symposium Series,
5. Fry SC, Smith RC, Renwick KF, Martin DJ, Hodge SK, Matthews KJ: Xyloglucan Endotransglycosylase, a new wall-loosening enzyme- activity from plants. Biochem J. 1992, 282: 821-828.
Cited by
164 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献