Selective spectrofluorimetric method for the determination of perindopril erbumine in bulk and tablets through derivatization with dansyl chloride

Abstract

Abstract Background Perindopril erbumine is an antihypertensive, which belongs to the category of angiotensin-converting enzyme inhibitors (ACE inhibitors) that inhibit the conversion of angiotensin I to angiotensin II. Methods A new, selective, and sensitive spectrofluorimetric method was developed for the determination of perindopril erbumine based on the reaction with dansyl chloride in alkaline medium to give a highly fluorescent derivative which was measured at 496 nm after excitation at 340 nm in dichloromethane. The reaction conditions were studied and optimized. Results Under the optimum conditions, the fluorescence intensity was linear over a concentration range of 1.0 to 21.0 μg/mL (R 2 = 0.9997) with a detection limit of 0.242 μg/mL. In order to validate the method, the results were compared with those obtained by a high performance liquid chromatography method. Conclusions The proposed method was successfully applied to the analysis of perindopril erbumine in pure form and tablets with good precision and accuracy as revealed by t- and F tests. The mechanism of the reaction has also been discussed.