Author:
Zhou Ying,Tang Yaoxiang,Luo Jiadi,Yang Yang,Zang Hongjing,Ma Jian,Fan Songqing,Wen Qiuyuan
Abstract
Abstract
Background
HSP60 is a heat shock proteins (HSPs) family member and help mitochondrial protein to fold correctly. Survivin is one of the inhibitors of apoptosis protein family member, which plays a significant part in cancer progression. They were capable of forming HSP60-survivin complexes and involved in the development of various tumors.
Methods
The Cancer Genome Atlas (TCGA) database demonstrated that HSP60 and survivin and their correlation on mRNA expression level with OSCC patients. Besides, expression of HSP60 and survivin proteins was studied utilizing immunohistochemistry in tissue microarrays (TMA) in OSCC and in adjacent non-cancerous squamous epithelium (Non-CCSE) tissues.
Results
Significantly increased levels of HSP60 and survivin in most cancers compared to normal tissue by pan-cancer analysis. HSP60 and survivin proved a significantly increased expression in OSCC samples compared to Non-CCSE both on mRNA and protein (both P < 0.05). Additionally, elevated HSP60 displayed a positive correlation with survivin in terms of mRNA and protein expression levels (all P < 0.001). Patients with OSCC who had advanced clinical stage or lymph node metastasis (LNM) showed higher HSP60 expression (P = 0.004, P = 0.006, respectively). Higher levels of the proteins HSP60 and survivin were significantly inversely correlated relationship with OSCC patients’ overall survival rates in multivariate survival analysis (P = 0.018, P = 0.040). From the above results, overexpression of HSP60 and survivin protein may serve as independent biomarkers predicting poor prognosis in OSCC.
Conclusions
Elevated HSP60 and survivin might be served as novel poor prognosis biomarkers for surgically resected OSCC patients.
Funder
Hunan Provincial Innovation Foundation for Postgraduate
the Fundamental Research Funds for the Central Universities of Central South University
the National Natural Science Foundation of China
Publisher
Springer Science and Business Media LLC
Reference37 articles.
1. Chang YT, Chu LJ, Liu YC, Chen CJ, Wu SF, Chen CH, Chang IY, Wang JS, Wu TY, Dash S et al. Verification of Saliva Matrix Metalloproteinase-1 as a strong diagnostic marker of oral Cavity Cancer. Cancers (Basel) 2020, 12(8).
2. Yao Y, Shen X, Zhou M, Tang B. Periodontal Pathogens promote oral squamous cell carcinoma by regulating ATR and NLRP3 inflammasome. Front Oncol. 2021;11:722797.
3. Alexandra T, Marina IM, Daniela M, Ioana SI, Maria B, Radu R, Maria TA, Tudor S, Maria G. Autophagy-A hidden but important actor on oral Cancer scene. Int J Mol Sci 2020, 21(23).
4. Yang M, Luo Q, Chen X, Chen F. Bitter melon derived extracellular vesicles enhance the therapeutic effects and reduce the drug resistance of 5-fluorouracil on oral squamous cell carcinoma. J Nanobiotechnol. 2021;19(1):259.
5. Lin F, Gao L, Su Z, Cao X, Zhan Y, Li Y, Zhang B. Knockdown of KPNA2 inhibits autophagy in oral squamous cell carcinoma cell lines by blocking p53 nuclear translocation. Oncol Rep. 2018;40(1):179–94.
Cited by
1 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献