LncRNAs ENST00000499459 and TCONS_00004989 enhance asthma progression in children with house dust mite-induced allergic asthma
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Published:2022-12-03
Issue:1
Volume:18
Page:
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ISSN:1710-1492
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Container-title:Allergy, Asthma & Clinical Immunology
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language:en
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Short-container-title:Allergy Asthma Clin Immunol
Author:
Xude Zhang,Shaojie Feng,Beibei Guo,Jingjing Liu,Donghua Xu,Fengxia Liu
Abstract
Abstract
Background
Long non-coding RNAs (lncRNAs) have been extensively reported to play critical roles in the pathogenesis of various disease, especially in cancer. However, little is known about the role of lncRNAs in the pathogenesis of pediatric allergic asthma.
Methods
High-throughput sequencing analysis was performed to identify differentially expressed mRNAs and lncRNAs in peripheral blood mononuclear cells (PBMCs) from 3 children with allergic asthma and 3 matched healthy controls. Bioinformatics analysis was used to select candidate lncRNAs and mRNAs that may be involved in the pathogenesis of asthma. Candidate lncRNAs were validated in a larger size of asthma patients and healthy controls. Finally, lncRNAs and molecular pathways associated with the pathogenesis of allergic asthma were identified by competing endogenous RNA (ceRNA) analysis.
Results
Five differentially expressed lncRNAs were identified after high-throughput sequencing and verified by real-time PCR. LncRNAs ENST0000631797, TCONS_00004989 and ENST00000499459 were verified to be differentially expressed in allergic asthma. Besides, ENST00000499459/DIXDC1 axis was identified to play a crucial role in allergic asthma after comprehensive ceRNA network analysis.
Conclusion
ENST00000499459 and TCONS_00004989 are potential biomarkers for house dust mite-induced allergic asthma.
Funder
Weifang Medical and Health Science and Technology Development Plan Project
Science and Technology Development Program of Weifang
National Natural Science Foundation, China
Natural Science Foundation, Shandong Province, China
Publisher
Springer Science and Business Media LLC
Subject
Insect Science,Ecology,Ecology, Evolution, Behavior and Systematics
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