Author:
Toseland Christopher P,McSparron Helen M,Flower Darren R
Abstract
Abstract
Background
Dehydrogenase enzymes belong to the oxidoreductase class and utilise the coenzymes NAD and NADP. Stereo-selectivity is focused on the C4 hydrogen atoms of the nicotinamide ring of NAD(P). Depending upon which hydrogen is transferred at the C4 location, the enzyme is designated as A or B stereospecific.
Description
The Dehydrogenase Stereospecificity Database v1.0 (DSD) provides a compilation of enzyme stereochemical data, as sourced from the primary literature, in the form of a web-accessible database. There are two search engines, a menu driven search and a BLAST search. The entries are also linked to several external databases, including the NCBI and the Protein Data Bank, providing wide background information. The database is freely available online at: http://www.jenner.ac.uk/DSD/
Conclusion
DSD is a unique compilation available on-line for the first time which provides a key resource for the comparative analysis of reductase hydrogen transfer stereospecificity. As databases increasingly form the backbone of science, largely complete databases such as DSD, are a vital addition.
Publisher
Springer Science and Business Media LLC
Subject
Applied Mathematics,Computer Science Applications,Molecular Biology,Biochemistry,Structural Biology
Reference21 articles.
1. You KS: Stereospecificity for nicotinamide nucleotides in enzymatic and chemical hydride transfer reactions. CRC Crit Review Biochem 1985, 17: 313–451.
2. You K, Arnold LJ, Allison WS, Kaplan NO: Enzyme stereospecificities for nicotinamide nucleotides. Trends Biochem Sci 1978, 3: 265. 10.1016/S0968-0004(78)95849-8
3. Levy HR, Talalay P, Vennesland B: The steric course of enzymatic reactions at the meso carbon atoms: Application of hydrogen isotopes. Progress in Stereochemistry 1962, 3: 299.
4. Cornforth JW, Cornforth RH, Donniger C, Popják G, Ryback G, Schroepfer GJ: B 163. Proc R Soc London 1965, B 163: 436.
5. Fisher HF, Ofner P, Conn EE, Vennesland B, Westheimer FH: Direct enzymatic transfer of hydrogen atoms between substrates and DPN. Fed Proc 1953, 11: 211.
Cited by
3 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献