DSD – An integrated, web-accessible database of Dehydrogenase Enzyme Stereospecificities

Author:

Toseland Christopher P,McSparron Helen M,Flower Darren R

Abstract

Abstract Background Dehydrogenase enzymes belong to the oxidoreductase class and utilise the coenzymes NAD and NADP. Stereo-selectivity is focused on the C4 hydrogen atoms of the nicotinamide ring of NAD(P). Depending upon which hydrogen is transferred at the C4 location, the enzyme is designated as A or B stereospecific. Description The Dehydrogenase Stereospecificity Database v1.0 (DSD) provides a compilation of enzyme stereochemical data, as sourced from the primary literature, in the form of a web-accessible database. There are two search engines, a menu driven search and a BLAST search. The entries are also linked to several external databases, including the NCBI and the Protein Data Bank, providing wide background information. The database is freely available online at: http://www.jenner.ac.uk/DSD/ Conclusion DSD is a unique compilation available on-line for the first time which provides a key resource for the comparative analysis of reductase hydrogen transfer stereospecificity. As databases increasingly form the backbone of science, largely complete databases such as DSD, are a vital addition.

Publisher

Springer Science and Business Media LLC

Subject

Applied Mathematics,Computer Science Applications,Molecular Biology,Biochemistry,Structural Biology

Reference21 articles.

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3. Levy HR, Talalay P, Vennesland B: The steric course of enzymatic reactions at the meso carbon atoms: Application of hydrogen isotopes. Progress in Stereochemistry 1962, 3: 299.

4. Cornforth JW, Cornforth RH, Donniger C, Popják G, Ryback G, Schroepfer GJ: B 163. Proc R Soc London 1965, B 163: 436.

5. Fisher HF, Ofner P, Conn EE, Vennesland B, Westheimer FH: Direct enzymatic transfer of hydrogen atoms between substrates and DPN. Fed Proc 1953, 11: 211.

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