Abstract
Abstract
Background
Virulence of the entomopathogenic nematode, Heterorhabditis indica against larvae (3rd, 4th and 5th larval instars) and pupae of citrus fruit piercing moth, Eudocima materna L. (Lepidoptera: Erebidae) was evaluated under laboratory conditions. The experiments included pathogenicity assessment of H. indica against larvae and pupae using a range of concentrations (10, 25, 50, 100 and 200 infective juveniles (IJs) larva−1 and pupa−1) as well as evaluation of their reproductive potential on different larval instars with different concentrations (50, 100, 200, 400 and 600 IJs larva−1).
Results
Application of increasing concentrations of IJs induced higher mortality on larval instars and pupae with mean mortality ranging from 26.6 to 100% (LC50- 3rd (14.43 IJs larva−1), 4th (17.08 IJs larva−1), 5th (23.63 IJs larva−1)) and 10–70% (LC50- 85.91 IJs pupa−1) after 48 h post-exposure, respectively. H. indica successfully reproduced in the 3rd, 4th and 5th larval instars of E. materna and their offspring emerged from the cadavers. The highest reproduction was recorded in 5th instar larvae (1,082,855 IJs larva−1) at 600 IJs larva−1 in E. materna.
Conclusion
The present study suggests that H. indica strain had the potential for biological control of E. materna.
Funder
Indian Council of Agricultural Research
Publisher
Springer Science and Business Media LLC
Subject
Insect Science,Plant Science,Agronomy and Crop Science,Ecology
Reference25 articles.
1. Acharya R, Hwang HS, Mostafiz MM, Yu YS, Lee KY (2020) Susceptibility of various developmental stages of the fall armyworm, Spodoptera frugiperda, to entomopathogenic nematodes. Insects 11:868. https://doi.org/10.3390/insects11120868
2. Aryal S, Nielsen UN, Sumaya NH, Wilson C, Riegler M (2022) Virulence, penetration rate and reproductive potential of entomopathogenic nematodes from eastern Australia in Queensland fruit fly, Bactrocera Tryoni. Biol Control 169:104871. https://doi.org/10.1016/j.biocontrol.2022.104871
3. Batalla-Carrera L, Morton A, García del Pino F (2010) Efficacy of entomopathogenic nematodes against the tomato leafminer Tuta absoluta in laboratory and greenhouse conditions. Biocontrol 55:523–530. https://doi.org/10.1007/s10526-010-9284-z
4. Blanco-Pérez R, Bueno-Pallero FÁ, Neto L, Campos-Herrera R (2017) Reproductive efficiency of entomopathogenic nematodes as scavengers. Are they able to fight for insect’s cadavers?. J Invertebr Pathol 148:1–9. https://doi.org/10.1016/j.jip.2017.05.003
5. Chhagan A, McKenna C (2019) Fruit-piercing moth, Eudocima phalonia (Linneaus, 1763) review: biology, ecology and pest management with reference to kiwifruit. In: A Plant & Food Research Report Prepared for Zespri International Limited; No. Milestone No. 77646. Contract No. 35746. Job code: P/310119/01. SPTS No. 17607; Plant and Food Research: Auckland, New Zealand.
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