Entomopathogenic efficacy of the endophytic fungi: Clonostachys sp. and Beauveria bassiana on Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae) larvae under laboratory and greenhouse conditions

Abstract

AbstractBackgroundEndophytic fungi are excellent sources of new natural bioactive products with potential for exploitation in medical, agricultural, and industrial fields. Beneficial effects of endophytes are not only limited to promoting plant growth, but also to react as plant protection agents against phytopathogenic microorganisms and plant pests. The tomato leaf miner,Tuta absoluta(Meyrick) (Lepidoptera: Gelechiidae) is one of the main tomato pests in South America and in the Mediterranean basin.ResultsIn the present work, the effectiveness of two endophytic fungiClonostachysspp. andBeauveria bassianawere tested againstT.absolutalarvae. Evaluation of the larvicidal effect in the laboratory by spraying 3 concentrations (D1= 4 × 107spores/ml,D2= 4 × 106spores/ml andD3= 4 × 105spores/ml) of fungal suspensions showed remarkable efficacy, which varied depending on the tested strain, concentration of application and time of exposure. Statistical analysis showed highly significant results on larval mortality rates for both endophytic than the control. After 15 days, dead larvae were mummified. Applications of tested isolates in greenhouse experiments confirmed their marked biological control efficacy than the synthetic insecticide (Emamectin benzoat). Number of mined leaves decreased depending on the contact time of the larvae with the fungal spores.ConclusionResults suggest that the two endophytic fungiB. bassianaandClonostachysspp. were very effective entomopathogenic fungi againstT. absoluta. Indeed, the present study revealed the pathogenicity ofClonostachysspp. againstT. absolutalarvae for the first time.