Abstract
Abstract
Background
The red palm weevil (RPW), Rhynchophorus ferrugineus (Coleoptera: Curculionidae), overruns date palm ranches in most Oases of Saudi Arabia and causes massive economic losses. Integrated pest management (IPM) of the RPW by biocontrol agents of bacterial isolates was the primary aim of the present study.
Results
Thirty-seven bacterial isolates were isolated from the larvae of R. ferrugineus, which were collected from infested Phoenix dactylifera trees growing in different parts of Saudi Arabia. 16S rDNA sequencing showed that the isolated bacteria were: Serratia marcescens, Klebsiella pneumoniae and Bacillus thuringiensis. The potential of these isolates was tested against R. ferrugineus in the laboratory. The use of these three isolates showed significant mortality percentages against R. ferrugineus. The highest reduction in mortality was recorded at the concentration of 1 × 108 CFU/ml; B. thuringiensis isolate displayed the highest potency in mortal percentage rate (100%), 4 days post-treatment, followed by S. marcescens isolate after 5 days, and then K. pneumoniae isolate after 6 days.
Conclusions
These results suggest that the use of these bacterial isolates was very useful in in vitro experiments, and it may consider those bacterial strains of interest as a potential biocontrol agent of R. ferrugineus.
Funder
Deanship of Scientific Research (DSR) at King AbdulAziz University, Jeddah, Saudi Arabia
Publisher
Springer Science and Business Media LLC
Subject
Insect Science,Plant Science,Agronomy and Crop Science,Ecology
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