Author:
Fanny Jumeau,Julien Sigala,Francisco-Jose Fernandez-Gomez,Sabiha Eddarkaoui,Sophie Duban-Deweer,Luc Buée,Hélène Béhal,Nicolas Sergeant,Valérie Mitchell
Abstract
Abstract
Background
The limitations of conventional sperm analyses have highlighted the need for additional means of evaluating sperm quality.
Methods
In a study of a cohort of 245 men with known conventional sperm parameters, one-dimensional PAGE was used to monitor protein content and quality in samples from individual ejaculates.
Results
The sperm protein content varied markedly from sample to another, especially in the high-molecular-weight range. The intensity of the 80–110 kDa bands was correlated with progressive motility (r = 0.15, p = 0.015) and was significantly higher (p = 0.0367) in the group of men with conventional parameters above the World Health Organization’s 2010 reference values than in the group with at least one subnormal parameter (i.e. semen volume, sperm concentration, sperm count per ejaculate, progressive motility, proportion of normal forms or multiple anomaly index below the lower reference value). Using mass spectrometry, the 80–110 kDa bands were found to correspond primarily to three proteins from the flagellum’s fibrous sheath: A-kinase anchor protein 4, A-kinase anchor protein 3, and spermatogenic cell-specific type 1 hexokinase.
Conclusion
One-dimensional PAGE constitutes a simple, rapid, reliable, inexpensive method for analyzing proteins associated with sperm motility in individual human ejaculates.
Funder
Lille University Hospital
Publisher
Springer Science and Business Media LLC
Subject
Urology,Reproductive Medicine
Cited by
5 articles.
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