Author:
Sun Shihui,Zhao Guangyu,Xiao Wenjun,Hu Jingya,Guo Yan,Yu Hong,Wu Xiaohong,Tan Yadi,Zhou Yusen
Abstract
Abstract
Background
The highly pandemic 2009 influenza A H1N1 virus infection showed distinguished skewed age distribution with majority of infection and death in children and young adults. Although previous exposure to related antigen has been proposed as an explanation, the mechanism of age protection is still unknown.
Methods
In this study, murine model of different ages were inoculated intranasally with H1N1 (A/Beijing/501/09) virus and the susceptibility and pathological response to 2009 H1N1 infection were investigated.
Results
Our results showed that the younger mice had higher mortality rate when infected with the same dose of virus and the lethal dose increased with age. Immunohistochemical staining of H1N1 antigens in mice lung indicated infection was in the lower respiratory tract. Most bronchial and bronchiolar epithelial cells in 4-week mice were infected while only a minor percentage of those cells in 6-month and 1-year old mice did. The young mice developed much more severe lung lesions and had higher virus load in lung than the two older groups of mice while older mice formed more inducible bronchus-associated lymphoid tissue in their lungs and more severe damage in spleen.
Conclusions
These results suggest that young individuals are more sensitive to H1N1 infection and have less protective immune responses than older adults. The age factor should be considered when studying the pathogenesis and transmission of influenza virus and formulating strategies on vaccination and treatment.
Publisher
Springer Science and Business Media LLC
Subject
Infectious Diseases,Virology
Reference43 articles.
1. Garten RJ, Davis CT, Russell CA, Shu B, Lindstrom S, Balish A, Sessions WM, Xu X, Skepner E, Deyde V, Okomo-Adhiambo M, Gubareva L, Barnes J, Smith CB, Emery SL, Hillman MJ, Rivailler P, Smagala J, de Graaf M, Burke DF, Fouchier RA, Pappas C, Alpuche-Aranda CM, Lopez-Gatell H, Olivera H, Lopez I, Myers CA, Faix D, Blair PJ, Yu C, et al.: Antigenic and genetic characteristics of swine-origin 2009 A(H1N1) influenza viruses circulating in humans. Science 2009, 325: 197-201. 10.1126/science.1176225
2. Statement to the press by WHO Director-General Dr Margaret Chan: World now at the start of 2009 influenza pandemic[http://www.who.int/mediacentre/news/statements/2009/h1n1_pandemic_phase6_20090611/en/]
3. Pandemic (H1N1) 2009 - update 93[http://www.who.int/csr/disease/swineflu/laboratory26_03_2010/en/]
4. Jain R, Goldman RD: Novel influenza A(H1N1): clinical presentation, diagnosis, and management. Pediatr Emerg Care 2009, 25: 791-796. 10.1097/PEC.0b013e3181c3c8f8
5. van den Brand JM, Stittelaar KJ, van Amerongen G, Rimmelzwaan GF, Simon J, de Wit E, Munster V, Bestebroer T, Fouchier RA, Kuiken T, Osterhaus AD: Severity of pneumonia due to new H1N1 influenza virus in ferrets is intermediate between that due to seasonal H1N1 virus and highly pathogenic avian influenza H5N1 virus. J Infect Dis 2010, 201: 993-999. 10.1086/651132
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