Author:
Ricci Patricia S,Schäfer Birgit,Kreil Thomas R,Falkner Falko G,Holzer Georg W
Abstract
Abstract
Modified vaccinia virus Ankara (MVA) has become a promising vaccine vector due to its immunogenicity and its proven safety in humans. As a general approach for stringent and rapid selection of recombinant MVA, we assessed marker rescue of the essential viral D4R gene in an engineered deletion mutant that is fully replication defective in wild-type cells. Recombinant, replicating virus was obtained by re-introduction of the deleted viral gene as a dominant selection marker into the deletion mutant.
Publisher
Springer Science and Business Media LLC
Subject
Infectious Diseases,Virology
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