Author:
Al-Shabanah Othman A,Hafez Mohamed M,Hassan Zeinab K,Sayed-Ahmed Mohamed M,Abozeed Waleed N,Al-Rejaie Salem S,Alsheikh Abdulmalik A
Abstract
Abstract
Background
Human papillomavirus (HPV) is associated with different malignancies but its role in the pathogenesis of ovarian cancer is controversial. This study investigated the prevalence, genotyping and physical state of HPV in ovarian cancer Saudi patients.
Methods
Hundred formalin fixed paraffin embedded (FFPE) ovarian carcinoma tissues and their normal adjacent tissues (NAT) were included in the study. HPV was detected by nested polymerase chain reaction (PCR) using degenerated HPVL1 consensus primer pairs MY09/MY11 and GP5+/GP6 + to amplify a broad spectrum of HPV genotypes in a single reaction. The HPV positive samples were further genotyped using DNA sequencing. The physical state of the virus was identified using Amplification of Papillomavirus Oncogene Transcripts (APOT) assay in the samples positive for HPV16 and/or HPV18.
Results
High percentage of HPV (42%) was observed in ovarian carcinoma compared to 8% in the NAT. The high-risk HPV types 16, 18 and 45 were highly associated with the advanced stages of tumor, while low-risk types 6 and 11 were present in NAT. In malignant tissues, HPV-16 was the most predominant genotype followed by HPV-18 and -45. The percentage of viral integration into the host genome was significantly high (61.1%) compared to 38.9% episomal in HPV positive tumors tissues. In HPV18 genotype the percentage of viral integration was 54.5% compared to 45.5% episomal.
Conclusion
The high risk HPV genotypes in ovarian cancer may indicate its role in ovarian carcinogenesis. The HPV vaccination is highly recommended to reduce this type of cancer.
Publisher
Springer Science and Business Media LLC
Subject
Infectious Diseases,Virology
Reference67 articles.
1. zur Hausen H: Papillomavirus infections--a major cause of human cancers. Biochim Biophys Acta 1996,1288(2):F55-78.
2. El-All HS, Refaat A, Dandash K: Prevalence of cervical neoplastic lesions and human papilloma virus infection in egypt: national cervical cancer screening project. Infect Agent Cancer 2007, 2: 12. 10.1186/1750-9378-2-12
3. Melo A, Montenegro S, Hooper T, Capurro I, Roa JC, Roa I: Human papilloma virus (HPV) typing in preneoplastic and neoplastic lesions of the uterine cervix in the IX region-Chile. Revista Med Chil 2003,131(12):1382-1390.
4. Wu QJ, Guo M, Lu ZM, Li T, Qiao HZ, Ke Y: Detection of human papillomavirus-16 in ovarian malignancy. Br J Cancer 2003,89(4):672-675. 10.1038/sj.bjc.6601172
5. Bosch FX, Lorincz A, Munoz N, Meijer CJ, Shah KV: The causal relation between human papillomavirus and cervical cancer. J Clin Pathol 2002,55(4):244-265. 10.1136/jcp.55.4.244
Cited by
44 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献