Author:
Xiang Jun,Ma Guangpeng,Zhang Shunchuan,Cheng Anchun,Wang Mingshu,Zhu Dekang,Jia Renyong,Luo Qihui,Chen Zhengli,Chen Xiaoyue
Abstract
Abstract
Knowledge of the intracellular location of a protein can provide useful insights into its function. Bioinformatic studies have predicted that the DEV pUL38 mainly targets the cytoplasm and nucleus. In this study, we obtained anti-pUL38 polyclonal sera. These antibodies were functional in western blotting and immunofluorescence in DEV-infected duck embryo fibroblasts (DEFs). pUL38 was expressed as a 51-kDa protein from 8 h post-infection onward, initially showing a diffuse distribution throughout the cytoplasm, and later in the nucleus. Furthermore, pUL38 was found in purified virus. These results provide the first evidence of the kinetics of expression and intracellular localization of DEV pUL38.
Publisher
Springer Science and Business Media LLC
Subject
Infectious Diseases,Virology
Reference21 articles.
1. Fadly AM, Glisson JR, McDougald LR, Nolan Lk, Swayne DE: Duck Virus Enteritis. In Diseases of Poultry. 12th edition. Edited by: Saif YM. American, Wiley-Blackwell; 2008:384-393.
2. Guo Yufei, Cheng Anchun, Wang Mingshu, Zhou Yi: Purification of anatid herpesvirus 1 particles by tangential-flow ultrafiltration and sucrose gradient ultracentrifugation. J Virol Methods 2009, 161: 1-6. 10.1016/j.jviromet.2008.12.017
3. Cheng Anchun, Wang Mingshu, Wen Ming, Zhou Weiguang, Guo Yufei, Jia Renyong, Xu Chao, Yuan Guiping, Liu YiChen: Construction of duck enteritis virus gene libraries and discovery, cloning and identification of viral nucleocapsid protein gene. High Technology Lett 2006, 16: 948-953.
4. Jia Renyong, Wang Mingshu, Cheng Anchun, Zhu Dekang, Ge Han, Xin Hongyi, Guo Yufei, Qi Xuefeng, Chen Xiaoyue: Cloning, expression, purification and characterization of UL24 partial protein of duck enteritis virus. Intervirology 2009, 52: 326-334. 10.1159/000242354
5. Xie Wei, Cheng Anchun, Wang Mingshu, Chang Hua, Zhu Dekang, Luo Qihui, Jia Renyong, Chen Xiaoyue: Expression and characterization of the UL31 protein from duck enteritis virus. Virol J 2009.,6(19):
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