Author:
Iqbal Tahir,Idrees Muhammad,Ali Liaqat,Hussain Abrar,Ali Muhammad,Butt Sadia,Yousaf Muhammad Zubair,Sabar Muhammad Farooq
Abstract
Abstract
Background
Pakistan is a highly endemic area for hepatitis E virus (HEV) infection. The aim of the current study was to isolate and characterize strains of HEV in two mini outbreaks.
Results
RNA was extracted and reverse transcribed to cDNA. Nested PCR was done for the detection of HEV RNA. The positive bands were eluted, cloned in TA vector and sequenced in both directions using genetic Analyzer (Applied Biosystem Inc, USA). Phylogenetic analysis was done using MEGA4 software. We isolated two new HEV genotype-1 strains from Lahore, Pakistan, based on cloning and sequencing of ORF2 region.
Conclusions
Our study suggest that both the human HEV strains were closely related to the Sar-55 but different from the Abb-2B and 87-Pakistan-B HEV isolates sharing 88-91% sequence identity to Pakistani isolate Sar-55. These results indicated that Sar-55 is the main endemic HEV strain in various areas of the country.
Publisher
Springer Science and Business Media LLC
Subject
Infectious Diseases,Virology
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