Author:
Song Cuiping,Zhu Chao,Zhang Chaofan,Cui Shangjin
Abstract
Abstract
A TaqMan-based real-time polymerase chain reaction (PCR) assay was devised for the detection of porcine parvovirus (PPV). Two primers and a TaqMan probe for the non-structural protein NS1 gene were designed. The detection limit was 1 × 102 DNA copies/μL, and the assay was linear in the range of 1 × 102 to 1 × 109 copies/μL. There was no cross-reaction with porcine circovirus 2 (PCV2), porcine reproductive and respiratory syndrome virus (PRRSV), pseudorabies virus (PRV), classical swine fever virus (CSFV), or Japanese encephalitis virus (JEV). The assay was specific and reproducible. In 41 clinical samples, PPV was detected in 32 samples with the real-time PCR assay and in only 11 samples with a conventional PCR assay. The real-time assay using the TaqMan-system can therefore be practically used for studying the epidemiology and management of PPV.
Publisher
Springer Science and Business Media LLC
Subject
Infectious Diseases,Virology
Reference10 articles.
1. Mengeling WL: Porcine parvovirus. In Diseases of Swine. Ninth edition. Edited by: Straw BE, Zimmerman JJ, D'Allaire S, Taylor DJ. Black-well Publishing, Ames, IA; 2006:93-1400.
2. Hong-Ying Chen, Xiao-Kang Li, Bao-An Cui, Zhan-Yong Wei, Xin-Sheng Li, Yan-Bin Wanga, Li Zhaoa, Zhen-Ya Wanga: A TaqMan-based real-time polymerase chain reaction for the detection of porcine parvovirus. Journal of Virological Methods 2009, 84-88.
3. Mengeling WL, Lager KM, Zimmerman JK, Samarikermani N, Beran GW: A current assessment of the role of porcine parvovirus as a cause of fetal porcine death. J Vet Diagn Invest 1991, 3: 33-35.
4. Kim J, Chae C: A comparison of virus isolation, polymerase chain reaction, immunohistochemistry, and in situ hybridization for the detection of porcine circovirus 2 and porcine parvovirus in experimentally and naturally coinfected pigs. J Vet Diagn Invest 2004,16(1):45-50.
5. Zeeuw EJ, Leinecker N, Herwig V, Selbitz HJ, Truyen U: Study of the virulence and cross-neutralization capability of recent porcine parvovirus field isolates and vaccine viruses in experimentally infected pregnant gilts. J Gen Virol 2007, 88: 420-427. 10.1099/vir.0.82302-0
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