Author:
Himeda Toshiki,Hosomi Takushi,Asif Naeem,Shimizu Hiroyuki,Okuwa Takako,Muraki Yasushi,Ohara Yoshiro
Abstract
Abstract
The pathogenicity of Saffold virus (SAFV) among humans still remains unclear, although it was identified as a novel human cardiovirus in 2007. In order to encourage the molecular pathogenetic studies of SAFV, we generated an infectious cDNA clone of SAFV type 3 (SAFV-3). The present study demonstrated that the synthesis of the full-length infectious RNA by T7 RNA polymerase was terminated by a homologous sequence motif with the human preproparathyroid hormone (PTH) signal in the SAFV-3 genome. To obtain the infectious RNA using T7 promoter, a variant of T7 RNA polymerase, which fails to recognize the PTH signal, was useful. This study will provide a valuable technical insight into the reverse genetics of SAFV.
Publisher
Springer Science and Business Media LLC
Subject
Infectious Diseases,Virology
Cited by
21 articles.
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