Author:
Hayta Sadiye,Smedley Mark A.,Demir Selcen U.,Blundell Robert,Hinchliffe Alison,Atkinson Nicola,Harwood Wendy A.
Abstract
Abstract
Background
Despite wheat being a worldwide staple, it is still considered the most difficult to transform out of the main cereal crops. Therefore, for the wheat research community, a freely available and effective wheat transformation system is still greatly needed.
Results
We have developed and optimised a reproducible Agrobacterium-mediated transformation system for the spring wheat cv ‘Fielder’ that yields transformation efficiencies of up to 25%. We report on some of the important factors that influence transformation efficiencies. In particular, these include donor plant health, stage of the donor material, pre-treatment by centrifugation, vector type and selection cassette. Transgene copy number data for independent plants regenerated from the same original immature embryo suggests that multiple transgenic events arise from single immature embryos, therefore, actual efficiencies might be even higher than those reported.
Conclusion
We reported here a high-throughput, highly efficient and repeatable transformation system for wheat and this system has been used successfully to introduce genes of interest, for RNAi, over-expression and for CRISPR–Cas9 based genome editing.
Funder
Biotechnology and Biological Sciences Research Council
Publisher
Springer Science and Business Media LLC
Subject
Plant Science,Genetics,Biotechnology
Cited by
122 articles.
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