Selection of reference genes for diurnal and developmental time-course real-time PCR expression analyses in lettuce
Author:
Funder
Biotechnology and Biological Sciences Research Council (GB)
Publisher
Springer Science and Business Media LLC
Subject
Plant Science,Genetics,Biotechnology
Link
http://link.springer.com/content/pdf/10.1186/s13007-016-0121-y
Reference45 articles.
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2. Gutierrez L, Mauriat M, Pelloux J, Bellini C, van Wuytswinkel O. Towards a systematic validation of references in real-time RT-PCR. Plant Cell. 2008;20(7):1734–5.
3. Gutierrez L, Mauriat M, Guenin S, Pelloux J, Lefebvre J, Louvet R, et al. The lack of a systematic validation of reference genes: a serious pitfall undervalued in reverse transcription-polymerase chain reaction (RT-PCR) analysis in plants. Plant Biotech J. 2008;6(6):609–18.
4. Bustin SA, Benes V, Nolan T, Pfaffl MW. Quantitative real-time RT-PCR—a perspective. J Mol Endocrinol. 2005;34(3):597–601.
5. Czechowski T, Stitt M, Altmann T, Udvardi MK, Scheible WR. Genome-wide identification and testing of superior reference genes for transcript normalization in Arabidopsis. Plant Physiol. 2005;139(1):5–17.
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