Author:
Saeed Fatima A.,M.Gumaa Mohammed,A.Abdelaziz Sana,Enan Khalid A.,Ahmed Selma K.,Hussien Mohammed O.
Abstract
Abstract
Background
Peste des Petits Ruminants (PPR) is a severe contagious viral disease, which mainly affects small ruminants. PPR is caused by a Morbillivirus that belongs to the family Paramyxoviridae. In this study 12 suspected PPR outbreaks among sheep and goats were investigated in four localities in Kassala State, Eastern Sudan, during 2015—2017. The causative agent was confirmed by a Sandwich Enzyme-Linked Immunosorbent Assay (sELISA), and a Reverse Transcription Polymerase Chain Reaction (RT-PCR) targeting a partial sequence of nucleocapsid protein gene (N- gene) and a partial sequence of fusion protein gene (F- gene). Sequencing and phylogenetic analysis were carried out on six N- gene based RT-PCR products selected from two outbreaks occurred on border and inner localities of Kassala State to determine the circulating lineages of PPRV strains. Identity percentages were determined between isolates in this study and previous Sudanese, and other (African and Asian) isolates which clustered along with them.
Results
Out of 30 samples, 22 (73.3%) were positive using sandwich ELISA. From 22 s ELISA positive samples, 17 (77.3%) were positive by Ngene based RT-PCR and only 7(43.8%) out of 16 positive samples by N gene based RT-PCR were positive using Fgene based RT-PCR. The sequencing and phylogenetic analysis confirmed involvement of the lineage IV of PPRV in outbreaks among small ruminants in Kassala State and high identity percentage between our isolates and previous Sudanese and other (African and Asian) isolates.
Conclusions
The present study demonstrates that genetic relationship between PPRV strains circulating in sheep in Kassala State, Eastern Sudan, and PPRV strains characterized as lineage IV in neighboring African countries such as Eretria,Ethiopia, Egypt, and other Asian countries
Funder
This study was funded by Animal Resources Research Corporation (ARRC), Ministry of Animal Resources, Khartoum, Sudan.
Publisher
Springer Science and Business Media LLC
Reference29 articles.
1. Office of International Epizootics (OIE). Manual of Diagnostic Tests and Vaccines for Terrestrial Animals Chapter 2.7.11: Peste des petits ruminants. Available from: https://www.oie.int/international-standard-Setting/terrestrialmanual/access-online, 2013
2. Kinne J, Kreutzer R, Kreutzer M, Wernery U, Wohlsein P. Peste des petits ruminants in Arabian wildlife. Epidemiology and infect. 2010;138(8):1211–4.
3. Murphy FA, Gibbs EP, Horsier MC, and Studdert, M J. Paramyxoviridae. In: Veterinary Virology. 3rded. Academic press, pp 423 U.S.A; 1999.
4. Gibbs EP, Taylor WP, Lawman MJ. Classification of Peste des Petits Ruminants Virus as the Fourth Member of the Genus Morbillivirus Intervirology. 1979;11(5):268–74.
5. Roeder P and Obi TU. Recognizing of Peste des petits ruminants, In: FAO Animal Health Manual (5). 28pp Publ. Food and Agriculture Organization of the United `Nations, Rome; 1999.
Cited by
2 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献