Real-time diagnostic analysis of MinION™-based metagenomic sequencing in clinical microbiology evaluation: a case report-Reference-Cited by-同舟云学术

Real-time diagnostic analysis of MinION™-based metagenomic sequencing in clinical microbiology evaluation: a case report

Published:2019-03-19 Issue:1 Volume:5 Page:
ISSN:2363-9024
Container-title:JA Clinical Reports
language:en
Short-container-title:JA Clin Rep

Author:

Tanaka Hiromasa,Matsuo Yoshiyuki,Nakagawa So,Nishi Kenichiro,Okamoto Akihisa,Kai Shinichi,Iwai Teppei,Tabata Yoshiteru,Tajima Takeshi,Komatsu Yuji,Satoh Motohiko,Kryukov Kirill,Imanishi Tadashi,Hirota Kiichi^ORCID

Funder

Japan Society for the Promotion of Science

the Japanese Society of Anesthesiologists

MEXT-Supported Program for the Strategic Research Foundation

Japan Agency for Medical Research and Development

Publisher

Springer Science and Business Media LLC

Subject

Anesthesiology and Pain Medicine

Link

http://link.springer.com/content/pdf/10.1186/s40981-019-0244-z.pdf

Reference7 articles.

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2. Rantakokko-Jalava K, Jalava J. Optimal DNA isolation method for detection of bacteria in clinical specimens by broad-range PCR. J Clin Microbiol. 2002;40:4211–7.

3. Didelot X, Bowden R, Wilson DJ, Peto TEA, Crook DW. Transforming clinical microbiology with bacterial genome sequencing. Nat Rev Genet. 2012;13:601–12. https://doi.org/10.1038/nrg3226 .

4. Mitsuhashi S, Kryukov K, Nakagawa S, Takeuchi JS, Shiraishi Y, Asano K, et al. A portable system for rapid bacterial composition analysis using a nanopore-based sequencer and laptop computer. Sci Rep. 2017;7:5657. https://doi.org/10.1038/s41598-017-05772-5 .

5. Kai S, Matsuo Y, Nakagawa S, Kryukov K, Matsukawa S, Tanaka H, et al. Rapid bacterial identification by direct PCR amplification of 16S rRNA genes using the MinIONTM nanopore sequencer. bioRxiv. 2018. https://doi.org/10.1101/435859 .

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