Author:
Singh Avtar,Benjakul Soottawat,Prodpran Thummanoon
Abstract
Abstract
Chitooligosaccharides (COS) from squid pen produced using amylase, lipase and pepsin were characterized. COS produced by 8% (w/w) lipase (COS-L) showed the maximum FRAP and ABTS radical scavenging activity than those prepared using other two enzymes. COS-L had the average molecular weight (MW) of 79 kDa, intrinsic viscosity of 0.41 dL/g and water solubility of 49%. DPPH, ABTS radical scavenging activities, FRAP and ORAC of COS-L were 5.68, 322.68, 5.66 and 42.20 μmol TE/g sample, respectively. Metal chelating activity was 2.58 μmol EE/g sample. For antibacterial activity, minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of COS-L against the targeted bacteria were in the range of 0.31–4.91 mg/mL and 0.62–4.91 mg/mL, respectively. Sardine surimi gel added with 1% (w/w) COS-L showed the lower PV, TBARS and microbial growth during 10 days of storage at 4 °C. COS-L from squid pen could inhibit lipid oxidation and extend the shelf-life of refrigerated sardine surimi gel.
Graphical abstract
Publisher
Springer Science and Business Media LLC
Reference50 articles.
1. Amiza, M., & Kang, W. (2013). Effect of chitosan on gelling properties, lipid oxidation, and microbial load of surimi gel made from African catfish (Clarias gariepinus). International Food Research Journal, 20, 1585.
2. Benjakul, S., Kittiphattanabawon, P., Sumpavapol, P., & Maqsood, S. (2014). Antioxidant activities of lead (Leucaena leucocephala) seed as affected by extraction solvent, prior dechlorophyllisation and drying methods. Journal of Food Science and Technology, 51, 3026–3037.
3. Buamard, N., & Benjakul, S. (2017). Ethanolic coconut husk extract: In vitro antioxidative activity and effect on oxidative stability of shrimp oil emulsion. European Journal of Lipid Science and Technology, 119, 1700131.
4. Carmen Gómez-Guillén, M., Montero, P., Teresa Solas, M., & Pérez-Mateos, M. (2005). Effect of chitosan and microbial transglutaminase on the gel forming ability of horse mackerel (Trachurus spp.) muscle under high pressure. Food Research International, 38, 103–110.
5. Choi, B.-K., Kim, K.-Y., Yoo, Y.-J., Oh, S.-J., Choi, J.-H., & Kim, C.-Y. (2001). In vitro antimicrobial activity of a chitooligosaccharide mixture against Actinobacillusactino mycetemcomitans and Streptococcus mutans. International Journal of Antimicrobial Agents, 18, 553–557.
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