Abstract
AbstractBacterial chondronecrosis with osteomyelitis (BCO) is a common cause of broiler lameness. Bacteria that are found in BCO lesions are intestinal bacteria that are proposed to have translocated through the intestinal epithelium and have spread systemically. One of the specific bacterial species frequently isolated in BCO cases is Enterococcus cecorum. In the current study, caecal isolates were obtained from birds derived from healthy flocks (12 isolates from 6 flocks), while isolates derived from caeca, colon, pericardium, caudal thoracic vertebrae, coxo-femoral joint, knee joint and intertarsal joint (hock) were obtained from broilers derived from BCO outbreaks (111 isolates from 10 flocks). Pulsed field gel electrophoresis was performed to determine similarity. Clonal E. cecorum populations were isolated from different bones/joints and pericardium from animals within the same flock, with intestinal strains carrying the same pulsotype, pointing to the intestinal origin of the systemically present bacteria. Isolates from the intestinal tract of birds from healthy flocks clustered away from the BCO strains. Isolates from the gut, bones/joints and pericardium of affected animals contained a set of genes that were absent in isolates from the gut of healthy animals, such as genes encoding for enterococcal polysaccharide antigens (epa genes), cell wall structural components and nutrient transporters. Isolates derived from the affected birds induced a significant higher mortality in the embryo mortality model as compared to the isolates from the gut of healthy birds, pointing to an increased virulence.
Funder
China Scholarship Council
Publisher
Springer Science and Business Media LLC
Reference34 articles.
1. Knowles TG, Kestin SC, Haslam SM, Brown SN, Green LE, Butterworth A, Pope SJ, Pfeiffer D, Nicol CJ (2008) Leg disorders in broiler chickens: prevalence, risk factors and prevention. PLoS One 3:e1545
2. Wideman RF, Prisby RD (2012) Bone circulatory disturbances in the development of spontaneous bacterial chondronecrosis with osteomyelitis: a translational model for the pathogenesis of femoral head necrosis. Front Endocrinol 3:183
3. McNamee PT, Smyth JA (2000) Bacterial chondronecrosis with osteomyelitis ('femoral head necrosis’) of broiler chickens: a review. Avian Pathol 29:477–495
4. Wideman RF Jr (2016) Bacterial chondronecrosis with osteomyelitis and lameness in broilers: a review. Poult Sci 95:325–344
5. Wideman RF Jr, Al-Rubaye A, Gilley A, Reynolds D, Lester H, Yoho D, Hughes JM, Pevzner I (2013) Susceptibility of 4 commercial broiler crosses to lameness attributable to bacterial chondronecrosis with osteomyelitis. Poult Sci 92:2311–2325
Cited by
3 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献