Potential genetic robustness of Prnp and Sprn double knockout mouse embryos towards ShRNA-lentiviral inoculation-Reference-Cited by-同舟云学术

Potential genetic robustness of Prnp and Sprn double knockout mouse embryos towards ShRNA-lentiviral inoculation

Published:2022-07-07 Issue:1 Volume:53 Page:
ISSN:1297-9716
Container-title:Veterinary Research
language:en
Short-container-title:Vet Res

Author:

Rau Andrea^ORCID,Passet Bruno,Castille Johan,Daniel-Carlier Nathalie,Asset Alexandre,Lecardonnel Jérome,Moroldo Marco,Jaffrézic Florence,Laloë Denis,Moazami-Goudarzi Katayoun,Vilotte Jean-Luc

Abstract

AbstractThe Shadoo and PrP prion protein family members are thought to be functionally related, but previous knockdown/knockout experiments in early mouse embryogenesis have provided seemingly contradictory results. In particular, Shadoo was found to be indispensable in the absence of PrP in knockdown analyses, but a double-knockout of the two had little phenotypic impact. We investigated this apparent discrepancy by comparing transcriptomes of WT, Prnp0/0 and Prnp0/0Sprn0/0 E6.5 mouse embryos following inoculation by Sprn- or Prnp-ShRNA lentiviral vectors. Our results suggest the possibility of genetic adaptation in Prnp0/0Sprn0/0 mice, thus providing a potential explanation for their previously observed resilience.

Funder

INRAE Animal Genetics department

Publisher

Springer Science and Business Media LLC

Subject

General Veterinary

Link

https://link.springer.com/content/pdf/10.1186/s13567-022-01075-4.pdf

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