Abstract
Abstract
Background
As core units of organ tissues, cells of various types play their harmonious rhythms to maintain the homeostasis of the human body. It is essential to identify the characteristics of cells in human organs and their regulatory networks for understanding the biological mechanisms related to health and disease. However, a systematic and comprehensive single-cell transcriptional profile across multiple organs of a normal human adult is missing.
Results
We perform single-cell transcriptomes of 84,363 cells derived from 15 tissue organs of one adult donor and generate an adult human cell atlas. The adult human cell atlas depicts 252 subtypes of cells, including major cell types such as T, B, myeloid, epithelial, and stromal cells, as well as novel COCH+ fibroblasts and FibSmo cells, each of which is distinguished by multiple marker genes and transcriptional profiles. These collectively contribute to the heterogeneity of major human organs. Moreover, T cell and B cell receptor repertoire comparisons and trajectory analyses reveal direct clonal sharing of T and B cells with various developmental states among different tissues. Furthermore, novel cell markers, transcription factors, and ligand-receptor pairs are identified with potential functional regulations in maintaining the homeostasis of human cells among tissues.
Conclusions
The adult human cell atlas reveals the inter- and intra-organ heterogeneity of cell characteristics and provides a useful resource in uncovering key events during the development of human diseases in the context of the heterogeneity of cells and organs.
Funder
National Natural Science Foundation of China
Guangdong Provincial Key Laboratory Construction Projection on Organ Donation and Transplant Immunology
Guangdong Provincial Natural Science Funds for Major Basic Science Culture Project
Guangdong Provincial Natural Science Funds for Distinguished Young Scholars
Special Support Program for Training High Level Talents in Guangdong Province
Pearl River Nova Program of Guangzhou
Science and Technology Program of Guangzhou
Guangdong Innovative and Entrepreneurial Research Team Program
Sun Yat-sen University Young Teacher Key Cultivate Project
National Program for Support of Top-Notch Young Professionals
Special Support Program of Guangdong
Chang Jiang Scholars Program
Publisher
Springer Science and Business Media LLC
Cited by
108 articles.
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