Author:
Sun Xiaoxue,Feng Daling,Liu Mengyang,Qin Ruixin,Li Yan,Lu Yin,Zhang Xiaomeng,Wang Yanhua,Shen Shuxing,Ma Wei,Zhao Jianjun
Abstract
Abstract
Background
Chinese cabbage (Brassica rapa ssp. pekinensis) experienced a whole-genome triplication event and thus has three subgenomes: least fractioned, medium fractioned, and most fractioned subgenome. Environmental changes affect leaf development, which in turn influence the yield. To improve the yield and resistance to different climate scenarios, a comprehensive understanding of leaf development is required including insights into the full diversity of cell types and transcriptional networks underlying their specificity.
Results
Here, we generate the transcriptional landscape of Chinese cabbage leaf at single-cell resolution by performing single-cell RNA sequencing of 30,000 individual cells. We characterize seven major cell types with 19 transcriptionally distinct cell clusters based on the expression of the reported marker genes. We find that genes in the least fractioned subgenome are predominantly expressed compared with those in the medium and most fractioned subgenomes in different cell types. Moreover, we generate a single-cell transcriptional map of leaves in response to high temperature. We find that heat stress not only affects gene expression in a cell type-specific manner but also impacts subgenome dominance.
Conclusions
Our study highlights the transcriptional networks in different cell types and provides a better understanding of transcriptional regulation during leaf development and transcriptional response to heat stress in Chinese cabbage.
Funder
Innovative Research Group Project of Hebei Natural Science Foundation
National Natural Science Foundation of China
Key R&D Project of Hebei
Natural Science Foundation of Hebei
Post-doctoral Science Foundation of China
Post-doctoral Science Foundation of Hebei
Science and Technology Department of Hebei
Publisher
Springer Science and Business Media LLC
Cited by
14 articles.
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