Author:
Liu Xin,Chen Yong,Zhang Yuannyu,Liu Yuxuan,Liu Nan,Botten Giovanni A.,Cao Hui,Orkin Stuart H.,Zhang Michael Q.,Xu Jian
Abstract
AbstractThe spatiotemporal control of 3D genome is fundamental for gene regulation, yet it remains challenging to profile high-resolution chromatin structure at cis-regulatory elements (CREs). Using C-terminally biotinylated dCas9, endogenous biotin ligases, and pooled sgRNAs, we describe the dCas9-based CAPTURE method for multiplexed analysis of locus-specific chromatin interactions. The redesigned system allows for quantitative analysis of the spatial configuration of a few to hundreds of enhancers or promoters in a single experiment, enabling comparisons across CREs within and between gene clusters. Multiplexed analyses of the spatiotemporal configuration of erythroid super-enhancers and promoter-centric interactions reveal organizational principles of genome structure and function.
Funder
National Institutes of Health
American Health Assistance Foundation
Welch Foundation
Cancer Prevention and Research Institute of Texas
Publisher
Springer Science and Business Media LLC
Cited by
41 articles.
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