Author:
He Yi-bo,Zhang Li,Zhou Lin-li,Chen Yi-min,Lu Jia-hong,Chen Jie,Liu Yong-lin
Abstract
Abstract
Objective
Women with an elevated basal FSH indicate diminished ovarian reserve and reduced oocyte and embryo numbers. DMSCs are likely to be involved in immune tolerance of pregnancy maintenance. We investigate the effect of follicle-stimulating hormones on the immunomodulatory functions of DMSCs.
Methods
DMSCs were primary cultured from decidual tissue. Pretreated DMSCs with mitomycin C, combined with CD4+ T lymphocytes, DMSCs + CD4+T co-culture system was established. Different physiological dose FSH (3 ng/ml,10 ng/ml,30 ng/ml,100 ng/ml) were used to co-culture system. Cytokines (IFN-γ, IL-2, IL-4, IL-6, IL-10, TNF-α) and other proteins (FSHR, MyD88) were measured.
Results
Compared with the control group (FSH (0 ng/mL) + CD4+T + DMSCs), the FSH concentration was 10, 30, and 100 ng/ml, IL-6 levels were significantly reduced (P < 0.05). IL-6, MyD88 protein expression was remarkably decreased (P < 0.05).
Conclusion
FSH/FSHR could negatively regulate the immunosuppressive function of DMSCs by reducing secretion of IL-6 levels through MyD88 pathways, but upstream and downstream signalling pathways require further validation.
Funder
Zhejiang Provincial Natural Science Foundation of China
Zhejiang Traditional Chinese Medicine Administration Project
Hainan Province Clinical Medical Center
Sanya Maternal and Child Health Hospital Golden Coconut Seeds
Sanya Special Science and Technology Program for Universities and Medical Institutions
Hainan Health Science Education Project
Publisher
Springer Science and Business Media LLC
Subject
Obstetrics and Gynecology,Oncology
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