Author:
Zhang Ju,Yang Jie,Yang Nan,Ma Jianfei,Lu Datong,Dong Yanhua,Liang Hao,Liu Dongjun,Cang Ming
Abstract
Abstract
Background
Obesity is a metabolic imbalance characterized by excessive deposition of white fat. The browning of white fat can effectively treat obesity and related diseases. Although Dlgap1 (Discs, Large (Drosophila) Homolog-Associated Protein 1) is suspected to have an effect on this process, no empirical evidence is available.
Methods
To understand the role of Dlgap1, we cultured white and brown fat cells, then performed overexpression and knockout experiments.
Results
We found that Dlgap1 overexpression in brown adipocytes inhibits brown-fat-related gene expression, promotes white-fat-related genes, while also increasing brown-adipocyte proliferation and apoptosis. However, the gene overexpression has no effect on brown adipocyte maturation. Knocking out Dlgap1 in white fat cells promotes the expression and inhibition of brown-fat-related and white-fat-related genes, respectively. Additionally, the knockout inhibits white fat cell proliferation and apoptosis, while also promoting their maturation.
Conclusions
Dlgap1 negatively regulates the browning of white adipocytes by influencing cell proliferation and apoptosis.
Funder
The Science and Technology Innovation Guided Project in Inner Mongolia Autonomous Region
Publisher
Springer Science and Business Media LLC
Subject
Biochemistry, medical,Clinical Biochemistry,Endocrinology,Endocrinology, Diabetes and Metabolism
Reference40 articles.
1. Lloydjones D, Adams R, Carnethon M, Simone GD, Ferguson TB, Flegal K, et al. Heart disease and stroke Statistics鈥9 update a report from the American Heart Association statistics committee and stroke statistics subcommittee. Circulation. 2009;119(3):e21–e181.
2. Mann JI. Diet and risk of coronary heart disease and type 2 diabetes. Lancet. 2002;360(9335):783–9.
3. Lee DH, Hong JE, Yun HM, Hwang CJ, Park JH, Han SB, et al. Interleukin-32Î2 ameliorates metabolic disorder and liver damage in mice fed high-fat diet. Obesity. 2015;23(3):615–22.
4. Virtanen K, Lidell M, Orava J, Heglind M, Westergren R, Niemi T, Taittonen M, et al. Functional brown adipose tissue in healthy adults. N Engl J Med. 2009;360(15):1518–25.
5. Natasa P, Walden TB, Shabalina IG, Timmons JA, Barbara C, Jan N. Chronic peroxisome proliferator-activated receptor gamma (PPARgamma) activation of epididymally derived white adipocyte cultures reveals a population of thermogenically competent, UCP1-containing adipocytes molecularly distinct from classic brown adipocyte. J Biol Chem. 2010;285(10):7153.
Cited by
3 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献